00185

Acetamide Nutrient Broth

NutriSelect^® Basic, suitable for microbiology

• Numéro MDL: MFCD00239573
• Code UNSPSC : 41171607
• Nomenclature NACRES : NA.85

Propriétés

• Stérilité: non-sterile
• Niveau de qualité: 100
• Gamme de produits: BioChemika
• Forme: powder
• Durée de conservation: limited shelf life, expiry date on the label
• Fabricant/nom de marque: NutriSelect^® Basic
• Technique(s): microbe id | utilization test: suitable
• pH final: 7.0±0.2 (25 °C)
• Application(s): bioburden testing; environmental; food and beverages; water monitoring; microbiology
• Adéquation: selective by defined nutrients for Pseudomonas spp.

Description

Application

Synthetic medium to detect microbial utilization of acetamide. Corresponds to the suggestions of the "Deutsches Institut für Normung (DIN)" and the test procedures set out in §35 LMBG for the examination of water for Pseudomonas aeruginosa.

Composants

Composition

Part A (Cat. No. 62192-109.4-KC)
Magnesium sulfate 0.158g/L
Sodium chloride 0.2g/L
Sodium molybdate 0.005g/L
Ferrous sulfate 0.0005g/L
Dipotassium hydrogen phosphate 0.2g/L

Part B (Cat. No. 00160-390.6G-KC)
Acetamide 2.0g/L

Notes préparatoires

Directions:
Dissolve 0.56 g Acetamide Nutrient Broth Part A and 2 g Acetamide in 950 ml distilled water. Check the pH and if necessary, adjust. Make up to 1 litre and pour into tubes. Autoclave at 121°C for 15 minutes.

Technique:
Medium is inoculated with a couple off loops from the culture to be assayed and it is incubated at 32-35°C for 24-48 hours before going to the isolation medium.
To confirm Ps. aeruginosa, inoculate a loop of culture in Asparagine Proline Broth (Cat. No. 17129) and incubate at 35-37°C for 24 hours. After this period, pour 1-2 drops of Nessler′s Reagent
(Cat. No. 72190) on to the culture and verify ammonia production: a change to yellow will mean ammonia production positive and therefore, Ps. aeruginosa presence.

Autres remarques

Decomposition of acetamide as taxonomic marker for some species of the genus Pseudomonas; Comparative studies on the enrichment of P. aeruginosa

Note de bas de page

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informations légales

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Warning
• Mentions de danger: H351
• Conseils de prudence: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classification des risques: Carc. 2
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges