NAD98-RO

β-Nicotinamide-adénine-dinucléotide hydrate

Grade II, free acid

• Synonyme(s) : β-DPN, β-NAD, Coenzyme 1, Cozymase, DPN, Diphosphopyridine nucléotide, NAD, Nadide
• Formule empirique (notation de Hill): C₂₁H₂₇N₇O₁₄P₂ · xH₂O
• Numéro CAS: 53-84-9
• Poids moléculaire : 663.43 (anhydrous basis)
• Numéro MDL: MFCD00036253
• Code UNSPSC : 41106305
• ID de substance PubChem : 329818700

Propriétés

• Pureté: 98% (dry weight)
• Forme: lyophilized
• Poids mol.: M_r 663.4
• Conditionnement: pkg of 1 g (10127981001); pkg of 10 g (10621650001); pkg of 5 g (10127990001)
• Fabricant/nom de marque: Roche
• Couleur: off-white
• pH: ~3.0 (50 mg/mL in water)
• λmax: 260 nm
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Chaîne SMILES: NC1=NC=NC2=C1N=CN2.O[C@@H]3[C@@H](COP(O)(OP(OC[C@@H](O4)[C@@H](O)[C@@H](O)[C@H]4[N+]5=CC=CC(C(N)=O)=C5)([O-])=O)=O)OC[C@@H]3O
• InChI: 1S/C21H27N7O14P2/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33/h1-4,7-8,10-11,13-16,20-21,29-32H,5-6H2,(H5-,22,23,24,25,33,34,35,36,37)/t10-,11-,13-,14-,15-,16-,20-,21-/m1/s1
• Clé InChI: BAWFJGJZGIEFAR-NNYOXOHSSA-N

Description

Description générale

Nicotinamide adenine dinucleotide (NAD) is a cofactor containing an adenylic acid and a nicotinamide-5′-ribonucleotide group linked together by a pyrophosphate moiety. It is ubiquitously found. In NAD⁺ complexes, the enzyme-cofactor interactions are highly conserved.

Application

NAD has been used in boiled-blood agar plate as a supplement for the growth of Actinobacillus pleuropneumoniae colony.

Actions biochimiques/physiologiques

Nicotinamide adenine dinucleotide (NAD) acts as an electron carrier and significantly participates in enzyme-catalyzed oxido-reduction processes and many genetic processes. NAD cycles between the oxidized (NAD⁺) and reduced (NADH) forms to maintain a redox balance necessary for continued cell growth. This cofactor is also involved in microbial catabolism. β-NAD acts as a substrate for various enzymes in several cellular processes.

Accepteur d'électrons

Conditionnement

Produit conditionné en fonction du poids solide.

Qualité

Absence of contaminants: LDH inhibitors are not detectable.
Organic solvents: <0.5% (GC)

Notes préparatoires

Storage conditions (working solution): An aqueous solution in a concentration of 10 mg/ml (pH 2.5 to 3) maintains > 99% of its NAD content after 8 weeks of storage at 2 to 8 °C.
The stability of an aqueous solution in a concentration of 40 mg/ml is not expected to differ from that of a solution in a concentration of 10 mg/ml.

An aqueous solution in a concentration of 50 mg/ml (pH 2.0) maintains 97% of its NAD content after 2 days, 94% after 6 days and 91% after 10 days of storage at 2 to 8 °C and about 83% after 10 days of storage at 33 °C.

An aqueous solution in a concentration of 1.38 mg/ml shows a decrease of NAD content of 4% after 8 weeks of storage at 2 to 8 °C, stored at -15 to -25 °C the stability is expected to be > 6 months. A solution of NAD in triethanolamine buffer (pH 5.0) maintians 90% of its NAD content after
10 days of storage at 2 to 8 °C and 80% after 10 days of storage at 33 °C.

Remarque sur l'analyse

Absorbance coefficients: 17.6 x 10³ l x mol^-1 x cm^-1 at 260 nm, 5.9 x 10³ l x mol^-1 x cm^-1 at 333 nm (CN complex)

ß-Nicotinamide adenine dinucleotide hydrate cofactor (NAD) has been used in an experimental protocol, done in order to mimic the citric acid cycle on an carbon electrode, in order to increase biofuel cell energy density.

Autres remarques

Il s′agit de la forme courante du NAD.

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable