BCIPP-RO

BCIP

99% (HPLC), powder, solubility: in DMF, soluble

• Synonyme(s) : 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, BCIP^® p-toluidine salt, X-phosphate p-toluidine salt
• Formule empirique (notation de Hill): C₈H₆BrClNO₄P · C₇H₉N
• Numéro CAS: 6578-06-9
• Poids moléculaire : 433.62
• Numéro MDL: MFCD00040636
• Code UNSPSC : 12352204
• ID de substance PubChem : 329774194

Propriétés

• Description: 5-bromo-4-chloro-3-indolyl-phosphate, 4-toluidine salt
• Niveau de qualité: 100
• Pureté: 99% (HPLC)
• Forme: powder
• Poids mol.: M_r 326.44 (BCIP); M_r 433.6 (BCIP toluidine salt)
• Conditionnement: pkg of 1 g (11585002001); pkg of 250 mg (10760994001)
• Fabricant/nom de marque: Roche
• Solubilité: DMF: soluble
• Température de stockage: 2-8°C
• Chaîne SMILES: Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
• InChI: 1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
• Clé InChI: QEIFSLUFHRCVQL-UHFFFAOYSA-N

Description

Description générale

BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.

Application

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

Qualité

Performance test: incubation with AP

Substrats

Histochemical substrate for alkaline phosphatase.

Remarque sur l'analyse

Absorption: Absorption maximum in the visible light is not determined.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations légales

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H312 - H315 - H319 - H335
• Conseils de prudence: P261 - P264 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P337 + P313
• Classification des risques: Acute Tox. 4 Dermal - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): 136.4 °F
• Point d'éclair (°C): 58 °C