11249738001
Roche
Phytohemagglutinin-L (PHA-L)
from Phaseolus vulgaris
Synonyme(s) :
PHA-L
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About This Item
Code UNSPSC :
23201100
Produits recommandés
Niveau de qualité
Stérilité
non-sterile; 0.2 μm filtered
Essai
>95% (SDS-PAGE)
Forme
lyophilized (clear, colorless solution after reconstitution)
Conditionnement
pkg of 5 mg
Fabricant/nom de marque
Roche
Application(s)
hematology
Température de stockage
2-8°C
Description générale
PHA-L is purified from Phaseolus vulgaris (red kidney bean) by standard chromatographic techniques.
Phytohemagglutinin (PHA), the lectin extract from the red kidney bean, consists of only L-type subunits (isolectin L4, "leuko-agglutinin"). It is an N-acetylgalactosamine/galactose sugar-specific lectin. PHA consists of five isolectins (L4E0, L3E1, L2E2, L1E3, L0E4) each being a tetramer held together by noncovalent forces. The subunits L (leukocyte reactive) have a high affinity for lymphocyte surface receptors, but little for those of erythrocytes, and are responsible for the mitogenic properties of the isolectins. E (erythrocyte reactive) is responsible for the erythrocyte-agglutinating properties.
Phytohemagglutinin (PHA), the lectin extract from the red kidney bean, consists of only L-type subunits (isolectin L4, "leuko-agglutinin"). It is an N-acetylgalactosamine/galactose sugar-specific lectin. PHA consists of five isolectins (L4E0, L3E1, L2E2, L1E3, L0E4) each being a tetramer held together by noncovalent forces. The subunits L (leukocyte reactive) have a high affinity for lymphocyte surface receptors, but little for those of erythrocytes, and are responsible for the mitogenic properties of the isolectins. E (erythrocyte reactive) is responsible for the erythrocyte-agglutinating properties.
Application
Phytohemagglutinin-L (PHA-L) consists of only L-type subunits (isolectin L4, "leuko-agglutinin"), and is well suited for high-efficiency induction and the functional analysis of T-lymphocytes. It is highly purified and tested for high-efficiency stimulation of human lymphocytes. It is also used as positive controls for peripheral blood mononuclear cells (PBMC) stimulation.
Actions biochimiques/physiologiques
Phytohemagglutinin (PHA) has mitogenic and potent cell agglutinating activities. It binds to T-cell membranes and stimulates cell division and metabolic activity. PHA has the ability to stimulate close contacts between cell membranes.
Caractéristiques
Biological activity: <5μg/ml for maximal stimulation of DNA synthesis (BrdU incorporation) with human peripheral blood lymphocytes
Séquence
PHA-L is a tetramer of 4 L-type subunits held together by noncovalent forces.
Notes préparatoires
Working concentration: 1 to 5 μg/ml
Approximately 1 to 5 μg/ml for the stimulation of human peripheral blood lymphocytes
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Approximately 1 to 5 μg/ml for the stimulation of human peripheral blood lymphocytes
Storage conditions (working solution): -15 to -25 °C
The reconstituted solution is stable at -15 to -25 °C.
Note: Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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