10354074001

Citrate Lyase (CL)

from Klebsiella pneumoniae (Aerobacter aerogenes)

• Synonyme(s) : Citrate Lyase (CL)
• Numéro de classification (Commission des enzymes): 4.1.3.6 (BRENDA, IUBMB)
• Code UNSPSC : 12352204

Propriétés

• Source biologique: bacterial (Klebsiella pneumoniae)
• Niveau de qualité: 100
• Forme: lyophilized
• Activité spécifique: ≥0.25 U/mg (At 25 °C with citrate as the substrate.)
• Conditionnement: pkg of 120 U
• Fabricant/nom de marque: Roche
• Technique(s): activity assay: suitable
• Couleur: light cream
• pH optimal: 8.0-9.0
• Solubilité: water: soluble
• Numéro d'accès NCBI: WP_323133469
• Numéro d'accès UniProt: P45413
• Application(s): life science and biopharma
• Activité étrangère: ICDH <0.05% (NAD as coenzyme); NADH oxidase <0.05%
• Température de stockage: 2-8°C

Description

Description générale

Citrate lyase is considered as a substrate- induced enzyme which helps in catalyzing reversible aldol cleavage of citrate to oxaloacetate and acetate. The enzyme is adaptive to anaerobic dissimilation of citrate.

Application

It was used in enzymatic assay to measure citrate concentration using microtiter plates.

Qualité

Contaminants: <0.05% ICDH (NAD specific) and “NADH oxidase”, each

Forme physique

Lyophilizate, stabilized with BSA, sucrose, MgSO₄ and EDTA. Note: A solution in double-distilled water has a pH-value of approximately 7.0. The enzyme can be relyophilized.

Notes préparatoires

Activator: Mg²⁺ (K_m = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn²⁺, Fe²⁺, Zn²⁺)

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash