SCT130
BioTracker 640 Red C1 Synaptic Dye
Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses.
Synonyme(s) :
Live cell imaging probe
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About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.47
Produits recommandés
Technique(s)
cell based assay: suitable
Méthode de détection
fluorometric
Conditions d'expédition
ambient
Description générale
BioTracker synaptic dyes are a series of fluorescent cationic styryl dyes developed to follow synaptic activities at neuromuscular junctions or synapses. These dyes typically have a lipophilic tail at one end and a highly hydrophilic at the other end. In the presence of cells or tissue preparations, the dyes partition between the aqueous phase, where the dyes are virtually non-fluorescent, and the outer leaflet of the cell surface membranes, where the dyes insert the lipophilic end into the membranes and become intensely fluorescent. During endocytosis following nerve stimulation, the dyes become trapped inside the vesicles. Thus, after washing off the dyes on the cell surface, the fluorescent signal is proportional to the number of newly formed vesicles. On the other hand, during exocytosis, the dyes are released from the vesicles along with neurotransmitters, causing a decrease in fluorescent signal. As a result, the change in fluorescent intensity reflects the amount of endocytosis/exocytosis or synaptic activity.
BioTracker 640 Red C1 Synaptic Dye is similar to BioTracker 640 Red C2(FM4-64) Synaptic Dye except that the lipophilic tail of is one carbon shorter and is expected to have a faster off-rate.
Spectral Properties
Absorbance (Membranes): 510 nm
Emission (Membranes): 750 nm
BioTracker 640 Red C1 Synaptic Dye is similar to BioTracker 640 Red C2(FM4-64) Synaptic Dye except that the lipophilic tail of is one carbon shorter and is expected to have a faster off-rate.
Spectral Properties
Absorbance (Membranes): 510 nm
Emission (Membranes): 750 nm
Application
Live cell fluorescent imaging
Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses.
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Live Cell Dye
Live Cell Dye
Qualité
Spectral Properties
Absorbance (Membranes): 510 nm
Emission (Membranes): 750 nm
Absorbance (Membranes): 510 nm
Emission (Membranes): 750 nm
Forme physique
Lyophilized
Stockage et stabilité
Store BioTracker 640 Red C1 Synaptic Dye at 2-8°C, protected from light. Stock solutions can be prepared at 10 mM and stored at 4°C or -20°C for six months or longer.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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