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Key Documents

SCR137

Sigma-Aldrich

Animal Free Collagenase, Type B

Animal Free Collagenase, Type B, is designed to have collagenase and secondary proteases similar to Types II collagenase.

Synonyme(s) :

MMP, Matrix metalloprotease

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About This Item

Code UNSPSC :
12352204
eCl@ss :
32160405
Nomenclature NACRES :
NA.54

Niveau de qualité

caseinase activity

≥300 caseinase units/mg

clostripain activity

≤5.0 clostripain units/mg

collagenase activity

≥15,000 U/vial

trypsin activity

≤0.5 trypsin units/mg

Technique(s)

cell culture | mammalian: suitable

Conditions d'expédition

wet ice

Description générale

Crude collagenases are widely used in enzymatic primary cell isolation and tissue dissociation procedures. Most researchers employ either crude collagenase preparations such as Types I, II, III, and IV or chromatographically purified collagenase. EMD Millipore’s Animal Free Collagenase, Type B, is prepared and purified from cultures grown in medium completely devoid of animal based components and designed for bioprocessing applications where introduction of animal derived pathogens, such as BSE,TSE and other mammalian viruses, must be prevented.
Product Source: Isolated and purified from Clostridium histolyticum cultures devoid of animal containing reagents.
This product is manufactured in an ISO9001:2008 certified enviroment.

Application

Animal Free Collagenase, Type B is designed to have collagenase and secondary proteases similar to Types II collagenase.
Animal Free Collagenase, Type B, is designed to have collagenase and secondary proteases similar to Types II collagenase.
Research Category
Stem Cell Research
Research Sub Category
Enzymes & Biochemistry

Forme physique

Product is diafiltered with water, filtered through a 0.22 micron membrane and lyophilized in vials.

Stockage et stabilité

Store at 2-8°C. Further dilutions should be made into appropriate buffer and/or medium to which protein has been added, such as DMEM/1% Human Serum Albumin or equivalent.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogrammes

Exclamation mark

Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Skin Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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