PF037

MMP-2, Proenzyme, Human, Recombinant, Mouse Cells

• Synonyme(s) : Gelatinase A, 72 kDa Gelatinase, Matrix Metalloproteinase 2
• Numéro de classification (Commission des enzymes): 3.4.24.24 (BRENDA, IUBMB)
• Code UNSPSC : 12352202

Propriétés

• Produit recombinant: expressed in mouse cell line
• Niveau de qualité: 100
• Pureté: ≥90% (SDS-PAGE)
• Forme: liquid
• Ne contient pas: preservative
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Conditions d'expédition: wet ice
• Température de stockage: −70°C

Description

Description générale

Recombinant, human pro-MMP-2 expressed in mouse cells. Can be used as a positive control for zymographic analysis, immunoblotting, and substrate cleavage assay. A portion (less than 10%) of the enzyme may be seen as a naturally-occurring dimer. Dimerization does not interfere with activation. May contain up to 10% TIMP proteins. During storage, a small portion (less than 10%) of the enzyme may also become activated.

Recombinant, human pro-MMP-2 expressed in mouse cells. Useful for immunoblotting, substrate cleavage and assay zymography. The ~72 kDa purified proenzyme MMP-2 may be used as a positive control or standard for zymographic analysis, immunoblotting, or substrate cleavage assays. Requires activation prior to use. Matrix metalloproteinases are members of a unique family of proteolytic enzymes that have a zinc ion at their active sites and can degrade collagens, elastin and other components of the extracellular matrix (ECM). These enzymes are present in normal healthy individuals and have been shown to have an important role in processes such as wound healing, pregnancy, and bone resorption. However, overexpression and activation of MMPs have been linked with a range of pathological processes and disease states involved in the breakdown and remodeling of the ECM. Such diseases include tumor invasion and metastasis, rheumatoid arthritis, periodontal disease and vascular processes such as angiogenesis, intimal hyperplasia, atherosclerosis and aneurysms. Recently, MMPs have been linked to neurodegenerative diseases such as Alzheimer′s, and amyotrophic lateral sclerosis (ALS). Natural inhibitors of MMPs, tissue inhibitor of matrix metalloproteinases (TIMPs) exist and synthetic inhibitors have been developed which offer hope of new treatment options for these diseases.



Regulation of MMP activity can occur at the level of gene expression, including transcription and translation, level of activation, or at the level of inhibition by TIMPs. Thus, perturbations at any of these points can theoretically lead to alterations in ECM turnover. Expression is under tight control by pro- and anti-inflammatory cytokines and/or growth factors and, once produced the enzymes are usually secreted as inactive zymograms. Upon activation (removal of the inhibitory propeptide region of the molecules) MMPs are subject to control by locally produced TIMPs1. All MMPs can be activated in vitro with organomercurial compounds (e.g., 4-aminophenylmercuric acetate), but the agents responsible for the physiological activation of all MMPs have not been clearly defined. Numerous studies indicate that members of the MMP family have the ability to activate one another13. The activation of the MMPs in vivo is likely to be a critical step in terms of their biological behavior, because it is this activation that will tip the balance in favor of ECM degradation. The hallmark of diseases involving MMPs appear to be stoichiometric imbalance between active MMPs and TIMPs, leading to excessive tissue disruption and often degradation. Determination of the mechanisms that control this imbalance may open up some important therapeutic options of specific enzyme inhibitors.

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In 150 mM NaCl, 20 mM Tris-HCl, 5 mM CaCl₂, 0.05% BRIJ^®-35 Detergent, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Remarque sur l'analyse

Specific Activity: Measured by its ability to degrade gelatin using zymography. 1 ng of enzyme is sufficient to visualize degraded gelatin by coomassie blue stain in zymography. Activated rhMMP-2 degrades type IV collagen, as visualized by SDS-PAGE and cleaves the coumarin peptide substrate, (7-methoxycoumarin-4-yl) Acetyl-Pro-Leu-Gly-Leu-(3-[2, 4-dinitrophenyl-L-2, 3-diaminopropionyl)-Ala-Arg-NH2 (Cat. No. 03-32-5032), as measured by fluorescence (excitation at 320 nm and emission at 405 nm).

Autres remarques

Buisson-Leqendre, N., et al. 2004. Arth. Rheumat.50, 2151.
Parsons, S.L., et al. 1997. Br. J. Surg.84, 160.
Backstrom, J.R., et al. 1996. J. Neuro.16, 7910.
Lim, G.P., et al. 1996. J. Neurochem.67, 251.
Xia, T., et al. 1996. Biochim. Biophys. Acta1293, 259.
Chandler, S. et al. 1995. Neurosci. Lett.201, 223.
Sang, Q.X., et al. 1995. Biochim. Biophys. Acta1251, 99.
Kenagy, R.D. and Clowes, A.W., 1994. In Inhibition of Matrix Metalloproteinases: Therapeutic Potential. Greenwald, R.A. and Golub L.M., Eds. 462.
Kleiner, D.E. and Stetler-Stevenson W.G., 1994. Anal. Biochem.218, 325.
Zempo, N., et al. 1994. J. Vasc. Surg.20, 209.
Birkedal-Hansen, H. 1993. J. Periodontol.64 474.
Stetler-Stevenson, W.G., et al. 1993. FASEB J.7, 1434.
Delaisse, J-M. and Vaes, G. 1992. In Biology and Physiology of the Osteoclast. B.R. Rifkin & C.V. Gay, Eds. 290.
Jeffrey, J.J. 1992. In Wound Healing: Biochemical and Clinical Aspects. R.F. Diegelmann and W.J. Lindblad, Eds. 177.
Jeffrey, J.J. 1991. Semin. Perinatol.15, 118.
Liotta, L.A., et al. 1991. Cell64, 327.
Harris, E. 1990. N. Engl. J. Med.322, 1277.
Heussen, C. and Dowdle, E.B., 1980. Anal. Biochem.102, 196.

Informations légales

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable