MABS270
Anti-PP2A reg. subunit B56 epsilon (PPP2R5E) Antibody, clone 5A5-1F3
clone 5A5-1F3, from mouse
Synonyme(s) :
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform, PP2A B subunit isoform B′-epsilon,
PP2A B subunit isoform B56-epsilon, PP2A B subunit isoform PR61-epsilon, PP2A B subunit isoform R5-epsilon
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
5A5-1F3, monoclonal
Espèces réactives
human, mouse
Technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotype
IgG1κ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... PTPA(5524)
Description générale
Protein phosphatase 2A (PP2A) is a family of ubiquitous heterotrimeric proteins that catalyze the removal of phosphate groups from serine and threonine residues. The PP2A enzymes contain core structural (A) and catalytic (C) subunits and variable regulatory B subunits. The B subunits regulate their catalytic activity, as well as dictate substrate specificity and subcellular localization. Recent studies have suggested that B56 epsilon subunits regulate p53-mediated apoptotic pathways, and can induce or suppress apoptosis depending on the cellular context. PP2A enzymes are also regulated by posttranslational modifications and endogenous inhibitors.
Spécificité
This antibody is specific for the B56 regulatory subunit of Protein phosphatase 2A (PP2A).
Immunogène
His-tagged recombinant protein corresponding to human PP2A reg. subunit B56 epsilon (PPP2R5E).
Application
Research Category
Neuroscience
Apoptosis & Cancer
Neuroscience
Apoptosis & Cancer
Research Sub Category
Kinases & Phosphatases
Apoptosis - Additional
Kinases & Phosphatases
Apoptosis - Additional
This Anti-PP2A reg. subunit B56 epsilon (PPP2R5E) Antibody, clone 5A5-1F3 is validated for use in Western Blotting, ICC, Immunoprecipitation for the detection of PP2A reg. subunit B56 epsilon (PPP2R5E).
Western Blot Analysis: A 1:1,000 dilution from a representative lot detected PP2A reg. subunit B56 epsilon (PPP2R5E) in 10 µg of U2OS cell lysate and mouse brain tissue lysate.
Immunocytochemistry Analysis: A representative lot detected PP2A reg. subunit B56 epsilon (PPP2R5E) in U2OS cells. (Max F. Perutz Laboratories, Medical University of Vienna, Austria).
Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous PP2A reg. subunit B56 epsilon (PPP2R5E) in a panel of U2OS cell lysates expressing the B56 alpha, beta, gamma, delta, and epsilon regulatory subunit of PP2A (Max F. Perutz Laboratories, Medical University of Vienna, Austria).
Immunocytochemistry Analysis: A representative lot detected PP2A reg. subunit B56 epsilon (PPP2R5E) in U2OS cells. (Max F. Perutz Laboratories, Medical University of Vienna, Austria).
Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous PP2A reg. subunit B56 epsilon (PPP2R5E) in a panel of U2OS cell lysates expressing the B56 alpha, beta, gamma, delta, and epsilon regulatory subunit of PP2A (Max F. Perutz Laboratories, Medical University of Vienna, Austria).
Qualité
Evaluted by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: A 1:1,000 dilution from a representative lot detected PP2A reg. subunit B56 epsilon (PPP2R5E) in 10 µg of NIH/3T3 cell lysate.
Western Blot Analysis: A 1:1,000 dilution from a representative lot detected PP2A reg. subunit B56 epsilon (PPP2R5E) in 10 µg of NIH/3T3 cell lysate.
Description de la cible
~50 kDa observed
Forme physique
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Remarque sur l'analyse
Control
NIH/3T3 cell lysate
NIH/3T3 cell lysate
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Ulrich Baumgartner et al.
Molecular cancer, 17(1), 44-44 (2018-02-20)
Epidermal growth factor receptor (EGFR) mutations enable constitutive active downstream signaling of PI3K/AKT, KRAS/ERK and JAK/STAT pathways, and promote tumor progression by inducing uncontrolled proliferation, evasion of apoptosis and migration of non-small cell lung cancer (NSCLC). In addition, such EGFR
Huan He et al.
eLife, 7 (2018-10-16)
The intracellular parasite Toxoplasma gondii, hijacks evolutionarily conserved host processes by delivering effector proteins into the host cell that shift gene expression in a timely fashion. We identified a parasite dense granule protein as GRA18 that once released in the
Qing Kang et al.
The Journal of biological chemistry, 289(25), 17480-17496 (2014-05-16)
The mitotic kinase Aurora A (AurA) is regulated by a complex network of factors that includes co-activator binding, autophosphorylation, and dephosphorylation. Dephosphorylation of AurA by PP2A (human, Ser-51; Xenopus, Ser-53) destabilizes the protein, whereas mitotic dephosphorylation of its T-loop (human
Jade J Dyson et al.
FASEB bioAdvances, 4(4), 273-282 (2022-04-14)
Protein phosphatase 2A (PP2A) is a heterotrimeric phosphatase that controls a wide range of cellular functions. The catalytic activity and intracellular location of PP2A are modulated by its association with regulatory B subunits, including B56 proteins, which are encoded by
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