MABS1270
Anti-Lipoprotein Lipase/LPL Antibody, clone 4-1a
clone 4-1a, from mouse
Synonyme(s) :
Lipoprotein lipase, hLPL, LPL
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
4-1a, monoclonal
Espèces réactives
human, mouse, bovine, rat
Technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotype
IgG2aκ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... LPL(4023)
Description générale
Lipoprotein lipase (EC 3.1.1.34; UniProt P06858; also known as hLPL, LPL) is encoded by the LPL (also known as LIPD) gene (Gene ID 4023) in human. Lipoprotein lipase (LPL) catalyzes the hydrolysis of triglycerides in plasma lipoproteins. LPL is produced by adipocytes and myocytes and secreted into the interstitial spaces, where it is bound by GPIHBP1 (a glycosylphosphatidylinositol-anchored protein of capillary endothelial cells) and shuttled to the luminal face of capillaries. The GPIHBP1–LPL complex is crucial for the binding of triglyceride-rich lipoproteins (TRLs) to endothelial cells and the subsequent lipolytic processing of TRLs. TRLs bind only the LPL-GPIHBP1 complex, but not GPIHBP1 alone, on the cell surface. A deficiency of either protein results in severe hypertriglyceridemia (chylomicronemia) and impaired delivery of lipid nutrients to parenchymal cells. LPL is produced with a signal peptide sequence (a.a. 1-27), the removal of which yields the mature 448-amino acid (a.a. 28-475) enzyme containing a PLAT (Polycystin-1, Lipoxygenase, Alpha-Toxin) domain (a.a. 341-464) and a heparin-binding domain (a.a. 346-441).
Spécificité
Clone 4-1a binds human and bovine LPL, as well as GPIHBP1-bound LPL, but not human hepatic lipase. Clone 4-1a does not inhibit LPL catalytic activity or LPL heparin interaction, nor does clone 4-1a compete against triglyceride-rich lipoproteins (LRLs) for bining GPIHBP1-LPL complex (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
Immunogène
Epitope: Near the N-terminus of mature LPL.
Purified human plasma lipoprotein lipase/LPL.
Application
Anti-Lipoprotein Lipase/LPL Antibody, clone 4-1a is an antibody against Lipoprotein Lipase/LPL for use in Immunohistochemistry (Paraffin), Western Blotting, ELISA, Immunocytochemistry, Immunofluorescence.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected lipoprotein lipase/LPL immunoreactivity in human pancreas, human skeletal muscle, and rat heart tissue sections.
Western Blotting Analysis: A representative lot detected human plasma lipoprotein lipase (LPL) and bovine milk LPL by Western blotting. Clone 4-1a exhibited much reduced binding to murine or chicken LPL, and failed to detect human hepatic lipase (hHL) (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
Western Blotting Analysis: A representative lot detected recombinant wild-type human LPL (rhLPL), as well as rhLPL with mutated Gln3 & Arg4. Clone 4-1a failed to react with rhLPL with mutated Ile8 or Asp9, nor rhLPL with either a.a. 5-15 or aa.16-25 deletion (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
ELISA Analysis: A representative lot was employed as the capture antibody for the detection of human LPL by sandwich ELISA, using biotinylated clone 5D2 as the detcting antibody (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
Immunocytochemistry Analysis: A representative lot detected the binding of human LPL to CHO and CHL cells transfected to express surface GPIHBP1, but not to mock transfected cells or cells transfected to express GPIHBP1 C65S mutant. Clone 4-1a does not compete against triglyceride-rich lipoproteins (TRLs) for binding the LPL-GPIHBP1 complex on cell surface (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
Immunofluorescence Analysis: A representative lot detected hLPL immunoreactivity associated with GPIHBP1 on capillary endothelial cells in OCT-embedded, methanol/acetone-fixed frozen skeletal muscle sections from mice expressing muscle-specific human LPL transgene. Much lower immunoreactivity of the endogenous mLPL was detected in skeletal muscle sections from non-transgenic mice (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
Note: Clone 4-1a exhibits much weaker affinity toward rat & murine LPL when compared with human LPL. Higher amount of sample loading (in Western blotting and ELISA) and/or high sensitivity detection method (e.g. HRP polymer or fluorescence detection in Western blotting and immunohistochemistry) is recommended when using this clone for detecting rat & murine LPL.
Western Blotting Analysis: A representative lot detected human plasma lipoprotein lipase (LPL) and bovine milk LPL by Western blotting. Clone 4-1a exhibited much reduced binding to murine or chicken LPL, and failed to detect human hepatic lipase (hHL) (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
Western Blotting Analysis: A representative lot detected recombinant wild-type human LPL (rhLPL), as well as rhLPL with mutated Gln3 & Arg4. Clone 4-1a failed to react with rhLPL with mutated Ile8 or Asp9, nor rhLPL with either a.a. 5-15 or aa.16-25 deletion (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
ELISA Analysis: A representative lot was employed as the capture antibody for the detection of human LPL by sandwich ELISA, using biotinylated clone 5D2 as the detcting antibody (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
Immunocytochemistry Analysis: A representative lot detected the binding of human LPL to CHO and CHL cells transfected to express surface GPIHBP1, but not to mock transfected cells or cells transfected to express GPIHBP1 C65S mutant. Clone 4-1a does not compete against triglyceride-rich lipoproteins (TRLs) for binding the LPL-GPIHBP1 complex on cell surface (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
Immunofluorescence Analysis: A representative lot detected hLPL immunoreactivity associated with GPIHBP1 on capillary endothelial cells in OCT-embedded, methanol/acetone-fixed frozen skeletal muscle sections from mice expressing muscle-specific human LPL transgene. Much lower immunoreactivity of the endogenous mLPL was detected in skeletal muscle sections from non-transgenic mice (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
Note: Clone 4-1a exhibits much weaker affinity toward rat & murine LPL when compared with human LPL. Higher amount of sample loading (in Western blotting and ELISA) and/or high sensitivity detection method (e.g. HRP polymer or fluorescence detection in Western blotting and immunohistochemistry) is recommended when using this clone for detecting rat & murine LPL.
Research Category
Signaling
Signaling
Research Sub Category
Lipid Metabolism & Weight Regulation
Lipid Metabolism & Weight Regulation
Qualité
Evaluated by Immunohistochemistry in mouse heart tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected lipoprotein lipase/LPL immunoreactivity in mouse heart tissue sections.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected lipoprotein lipase/LPL immunoreactivity in mouse heart tissue sections.
Description de la cible
53.16 kDa (ProLPL) and 50.39 (Mature LPL) calculated. ~56 kDa observed (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
Forme physique
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique