MABN1840

Anti-Neuritin (CPG15) Antibody, clone A6.1

clone A6.1, from mouse

• Synonyme(s) : candidate plasticity gene 15, CPG15
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: A6.1, monoclonal
• Espèces réactives: rat, human
• Réactivité de l'espèce (prédite par homologie): monkey (based on 100% sequence homology), bovine (based on 100% sequence homology)
• Conditionnement: antibody small pack of 25 μg
• Technique(s): western blot: suitable
• Isotype: IgG2bκ
• Numéro d'accès NCBI: NP_001265639
• Numéro d'accès UniProt: Q9NPD7
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... NRN1(51299)

Description

Description générale

Neuritin (UniProt: Q9NPD7) is encoded by the NRN1 (also known as NRN) gene (Gene ID: 51299) in human. Neuritin, a conserved, glycosylphosphatidylinositol (GPI)-anchored protein that plays a role in the development, survival and function of neurons. Its activity is induced by brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3). It is highly expressed by induced and natural regulatory T cells (TREGs). It also promotes the maintenance of the TREGs pool by stabilizing Foxp3 expression and promoting the persistence of these cells. Deficiency of Neuritin is shown to result in an imbalance between central and peripheral TREG populations and functions. Neuritin has a wide variety of effects in the nervous system, such as promoting neurite outgrowth, modulating neurite outgrowth during neuronal differentiation, and protecting motor neuron axons. It can also promote dendritic growth, regulate synaptic plasticity, and stabilize active synapses, and promote synaptic maturation. Its expression in the hippocampus is shown to be induced by neuronal activity following chemical- or electrical-induced seizures, ischemia, and exercise. Chronic antidepressant treatment is reported to increase neuritin expression in rat brain. Neuritin is synthesized with a signal peptide (aa 1-27) and a propeptide (aa 28-116) that are cleaved off in the mature form (aa 117-142). (Ref.: Son, H., et al. (2012). Proc. Natl. Acad. Sci. USA. 109(28); 11378-83; Zhou, S., and Zhou, J (2014). Curr. Med. Chem. 21(10); 1212-19). Note: A6.1 hybridoma clone, and WT/KO validation, courtesy of Lauren Makuch and Dr. Elly Nedivi, Massachusetts Institute of Technology.

Spécificité

Clone A6.1 detects human and rat Neuritin.

Immunogène

GST-tagged full-length human recombinant Neuritin.

Application

Anti-Neuritin (CPG15), clone A6.1, Cat. No. MABN1840, is a highly specific mouse monoclonal antibody that targets Neuritin and has been tested for use in Western Blotting.

Research Category
Neuroscience

Qualité

Evaluated by Western Blotting with recombinant human/rat neuritin.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected recombinant human/rat Neuritin (CPG15).

Description de la cible

~15 kDa observed; 15.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.