MABN1571

Anti-ARHGEF9 Antibody, clone 4D7.1

clone 4D7.1, from mouse

• Synonyme(s) : Rho guanine nucleotide exchange factor 9, Collybistin, PEM-2 homolog, Rac/Cdc42 guanine nucleotide exchange factor 9
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 4D7.1, monoclonal
• Espèces réactives: mouse
• Réactivité de l'espèce (prédite par homologie): human (based on 100% sequence homology)
• Technique(s): western blot: suitable
• Isotype: IgG2bκ
• Numéro d'accès NCBI: NP_056000
• Numéro d'accès UniProt: O43307
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ARHGEF9(23229)

Description

Description générale

Rho guanine nucleotide exchange factor 9 (UniProt O43307; also known as Collybistin, PEM-2 homolog, Rac/Cdc42 guanine nucleotide exchange factor 9) is encoded by the ARHGEF9 (also known as ARHDH9, EIEE8, KIAA0424) gene (Gene ID 23229) in human. Guanine nucleotide exchange factors (GEFs) are cytoplasmic proteins that activate GTPases by stimulating the release of guanosine diphosphate (GDP) to allow binding of guanosine triphosphate (GTP). ARHGEF9 acts as a specific GEF for CDC42 and promotes formation of GPHN clusters. In the brain tissue it interacts with the receptor-anchoring protein gephyrin to activate dc42, which is known to regulate actin cytoskeleton dynamics. Its binding to gephyrin can also modulate GABAergic and glycinergic neurotransmission at the synapse. Abnormalities in its expression have been linked to epileptic encephalopathy, early infantile, 8.

Spécificité

Clone 4D7.1 targets ARHGEF9 C-terminal region present in human isoforms 1/2/3 and mouse isoforms 1/2/4 reported by UniProt (O43307 and Q3UTH8). Reactivity toward mouse isoform 3 is not expected.

Immunogène

GST-tagged human ARHGEF9 C-terminal fragment.

Application

Anti-ARHGEF9 Antibody, clone 4D7.1, Cat. No. MABN1571, is a highly specific mouse monoclonal antibody that targets ARHGEF9 and has been tested in Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A 1:500 dilution from a representative lot detected ARHGEF9 in 10 µg of mouse whole brain tissue lysate.

Qualité

Evaluated by Western Blotting in mouse hypothalamus tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected ARHGEF9 in 10 µg of mouse hypothalamus tissue lysate.

Description de la cible

~61 kDa observed. 60.98/54.97/49.46 kDa (human isoform 1/2/3) and 60.93/58.62/60.86 kDa (mouse isoform 1/2/4) calculated. Human isoforms 1 and 2 are also known as CB3(SH3+) and CB3(SH3-). Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Protein G purified.

Purified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1