MABN1545

Anti-MRP2 Antibody, clone 4C9.2

clone 4C9.2, from mouse

• Synonyme(s) : Canalicular multispecific organic anion transporter 1, ATP-binding cassette sub-family C member 2, Canalicular multidrug resistance protein, cMRP, Multidrug resistance-associated protein 2
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 4C9.2, monoclonal
• Espèces réactives: human
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG2bκ
• Numéro d'accès NCBI: NP_000383
• Numéro d'accès UniProt: Q92887
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ABCC2(1244)

Description

Description générale

Canalicular multispecific organic anion transporter 1 (UniProt Q92887; also known as ATP-binding cassette sub-family C member 2, Canalicular multidrug resistance protein, cMRP, Multidrug resistance-associated protein 2) is encoded by the ABCC2 (also known as CMOAT, CMOAT1, CMRP, DJS, MRP2) gene (Gene ID 1244) in human. MRP2/ABCC2 belongs to the family of human ATP-binding cassette (ABC) transporters that are classified into seven subfamilies from ABC-A to ABC-G based on their sequence similarities. P-glycoprotein (P-gp/ABCB1), MRP2/ABCC2, and breast cancer resistance protein (BCRP/ABCG2) are well known plasma membrane ABC transporters that mediate the export of intracellular drugs, drug conjugates and metabolites. MRP2/ABCC2 is the second member of the ABCC subfamily of 13 transporters (ABCC1 to ABCC13). MRP2 is expressed in the apical hepatocyte plasma membrane, renal proximal tubules and small intestine, where it plays a role in the elimination and oral bioavailability of drugs, xenotoxins and their metabolites. MRP2 primarily functions as an organic anion transporter to mediate the elimination of bile acids, GSH and conjugated metabolites of numerous drugs and other xenobiotics. Weakly basic drugs appear to be co-transported with GSH by MRP2. Methotrexate, anthracyclines (doxorubicin, epirubicin), mitoxantrone, cisplatin, and etoposide are well known chemotherapeutic substrates of MRP2. MRP2 contains 17-transmembrane helices (a.a. 28-48, 69-89, 94-114, 127-147, 166-186, 314-334, 361-381, 438-458, 462-482, 545-565, 588-608, 972-992, 1034-1054, 1098-1118, 1120-1140, 1212-1232, 1235-1255), having its N-terminal end (a.a. 1-27) exposed extracellularly and C-terminal end (a.a. 1256-1545) at the cytoplasmic side. Two conserved nucleotide-binding domains (NBDs; a.a. 671-678 and 1334-1341) mediate the binding and hydrolyzing ATP via an ATPase, thereby providing energy for substrates export from the cytoplasm to the extracellular space.

Spécificité

Clone 4C9.2 targets an epitope within the third cytoplasmic domain of human MRP2.

Immunogène

GST-tagged recombinant fragment corresponding to the third cytoplasmic domain of human MRP2.

Application

Anti-MRP2, clone 4C9.2, Cat. No. MABN1545, is a highly specific mouse monoclonal antibody, that targets ABCC2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected MRP2 in human liver tissue sections.

Research Category
Neuroscience

Qualité

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected MRP2 in 10 µg of HepG2 cell lysate.

Description de la cible

~175-260 kDa observed. 174.2 kDa calculated. Larger-than-calculated sizes and smeary banding pattern are consistent with the detection of differentially glycosylated MRP2 species. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Protein G purified.

Purified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable