MABN1101

Anti-SRY, clone 1G4 Antibody

clone 1G4, from mouse

• Synonyme(s) : Sex-determining region Y protein, Testis-determining factor, SRY
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1G4, monoclonal
• Espèces réactives: human
• Technique(s): ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG1
• Numéro d'accès UniProt: Q05066
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SRY(6736)

Description

Description générale

Sex-determining region Y protein, also known as Testis-determining factor, and encoded by the human gene SRY or TDF is a transcriptional regulator that controls a genetic switch in male development. SRY is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) to Sertoli rather than granulosa cells. In male adult brain SRY is involved in the maintenance of motor functions of dopaminergic neurons. SRY is also involved in different aspects of gene regulation including promoter activation or repression, DNA bending and manipulation. The SRY HMG box recognizes DNA by partial intercalation into the minor groove, and SRY is also involved in pre-mRNA splicing. SRY is expressed in the developing testis and surrounding tissue and can be found expressed in neurons in the adult. SRY is nuclear associated.

Immunogène

Purified recombinant fragment of human SRY expressed in E. Coli

Application

Anti-SRY, clone 1G4 Antibody is an antibody against SRY for use in western blotting, IHC, flow cytometry & ELISA.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected SRY in cervical and ovarian cancer tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected SRY in HepG2 cells.

ELISA: A representative lot detected control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Qualité

Evaluated by Western Blotting in NTERA-2 cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected SRY in NTERA-2 cell lysate.

Description de la cible

~36 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal in PBS containing up to 0.1% sodium azide and 0.5% protein stabilizer.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable