MABF2767

Anti-MERS-CoV NP Antibody

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: #20, monoclonal
• Poids mol.: calculated mol wt 25.05 kDa; observed mol wt ~45 kDa
• Espèces réactives: virus
• Conditionnement: antibody small pack of 100 μg
• Technique(s): ELISA: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG2b
• Numéro d'accès UniProt: R9UM87
• Conditions d'expédition: dry ice
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: Unknown ... N nucleocapsid protein(14254601)

Description

Description générale

Nucleoprotein (UniProt: R9UM87; also known as Nucleocapsid protein, NC, Protein N) is encoded by the N gene (Gene ID: 14254601) in Middle East respiratory syndrome-related coronavirus (MERS-CoV). The MERS-CoV is a positive-strand RNA virus of the betacoronavirus genus within the Coronaviridae family that causes severe respiratory syndrome in human. The mature MERS-CoV contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). Nucleoprotein of MERS-CoV is a homooligomeric multifunctional protein that plays roles in genome encapsidation, RNA synthesis during replication, host translational shutoff, and innate immunity disruption. It packages the positive strand viral genome RNA into helical ribonucleocapsid and is involved in virion assembly through its interactions with the viral genome and membrane protein M. The nucleoprotein is reportedly organized into two folded domains known as the N-terminal domain (NTD) and the C-terminal domain (CTD) that are separated by a disordered region (LKR) containing a serine/ arginine stretch. Clone 46 displays specificity for MERS-CoV nucleoprotein and does not show cross-reactivity with nucleoprotein from other related viruses, including SARS coronavirus. (Ref.: Nguyen, THV., et al. (2019). Acta Crystallogr. D. Struct. Biol. 75(1); 8-15; Yamaoka, Y., et al. (2016). Front. Microbiol. 7; Article 509).

Spécificité

Clone 20 is a mouse monoclonal antibody that detects nucleoprotein of Middle East respiratory syndrome-related coronavirus (MERS-CoV). It target an epitope within 6 amino acids from the C-terminal region.

Immunogène

His-tagged recombinant fragment corresponding to 292 amino acids (122-413) from the nucleoprotein of Middle East respiratory syndrome-related coronavirus (MERS-CoV).

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant MERS-CoV nucleoprotein

Western Blotting Analysis: A 1:1,000,000 dilution of this antibody detected recombinant Middle East respiratory syndrome-related coronavirus (MERS-CoV) Nucleoprotein.

Tested Applications

Western Blotting Analysis: A representative lot detected MERS-CoV NP in Western Blotting applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).

Immunoprecipitation Analysis: A representative lot immunoprecipitated MERS-CoV NP in Immunoprecipitation applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).

ELISA Analysis: A representative lot detected MERS-CoV NP in ELISA applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-MERS-CoV NP, clone 20, Cat. No. MABF2767, is a mouse monoclonal antibody that detects nucleoprotein in Middle East respiratory syndrome-related coronavirus (MERS-CoV) and is tested for use in ELISA, Immunoprecipitation, and Western Blotting.

Forme physique

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable