MABF2005

Anti-CCR5 (CD195) Antibody, clone HEK/1/85a/7a

clone HEK/1/85a/7a, from rat

• Synonyme(s) : C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, HIV-1 fusion coreceptor, CD195
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: rat
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: HEK/1/85a/7a, monoclonal
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μg
• Technique(s): flow cytometry: suitable; immunocytochemistry: suitable
• Isotype: IgG2bκ
• Numéro d'accès NCBI: NP_000570.1
• Numéro d'accès UniProt: P51681
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CCR5(1234)

Description

Description générale

C-C chemokine receptor type 5 (UniProt: P51681; also known as C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, HIV-1 fusion coreceptor, CD195) is encoded by the CCR5 (also known as CMKBR5) gene (Gene ID: 1234) in human. CCR-5 is a multi-pass membrane protein that is expressed in memory T-cells, macrophages, and immature dendritic cells, and is upregulated by proinflammatory cytokines. It is also shown to be highly expressed in spleen, thymus, and on THP-1 and KG-1a cells. Lower expression is observed in ovary and lung. CCR-5 serves as a receptor for a number of inflammatory CC-chemokines and subsequently transduces a signal by increasing intracellular calcium levels. It also plays a role in the control of granulocytic lineage proliferation or differentiation. CCR-5 is acts as a co-receptor (CD4 being the primary receptor) of HIV-1 and HIV-2. It also couples to Gi class of G-proteins to inhibit cAMP production and stimulate calcium release. CCR-5 undergoes sulfation on two tyrosine residues in the N-terminal region, which increases efficiency of HIV-1 entry into cells. It also undergoes phosphorylation at C-terminal serine residues following binding to CC chemokines, especially APO-RANTES. Mutations in CCR5 gene have been linked to insulin-dependent diabetes mellitus. (Ref.: Blanpain, C., et al. (2002). Receptors Channels. 8(1);19-31).

Spécificité

Clone HEK/1/85a/7a is a rat monoclonal antibody that specifically detects human C-C-chemokine receptor 5 (CD195).

Immunogène

CHO cells transfected with full length human CCR-5 (CD195).

Application

Anti-CCR5 (CD195), clone HEK/1/85a/7a, Cat. No. MABF2005, is a rat monoclonal antibody that detects C-C chemokine receptor type 5 (CCR5) and has been tested for use in Flow Cytometry and Immunocytochemistry.

Immunocytochemistry Analysis: A representative lot detected CCR5 (CD195) in Immunocytochemistry applications (Mueller, A., et. al. (2002). Blood. 99(3):785-91).

Flow Cytometry Analysis: A representative lot detected CCR5 (CD195) in Flow Cytometry applications (Mueller, A., et. al. (2002). Blood. 99(3):785-91).

Qualité

Evaluated by Flow Cytometry in wrote RBL-2H3 cells stably transfection with CCR5.

Flow Cytometry Analysis (FC): 1 µg of this antibody detected C-C chemokine receptor type 5 (CD195) in one million CCR-5 transfected RBL-2H3 cells.

Description de la cible

40.52 kDa calculated.

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to lot specific datasheet.