MABE254

Anti-Protein-arginine deiminase 4 Antibody, clone 10F1

clone 10F1, from mouse

• Synonyme(s) : HL-60 PAD, Protein-arginine deiminase type-4, Peptidylarginine deiminase IV, Protein-arginine deiminase type IV
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 10F1, monoclonal
• Espèces réactives: human
• Technique(s): ChIP: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_036519
• Numéro d'accès UniProt: Q9UM07
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PADI4(23569)

Description

Description générale

Protein-arginine deiminase type-4 (PAD4; formerly PAD5) is one of five PAD (1-4, 6) homologues that belong to the superfamily of multidomain amidinotransferases. The PAD enzymes catalyze deiminating reactions in which guanidinium is hydrolyzed to peptidylcitrulline. PAD4 is a calcium-regulated nuclear-localized enzyme. It modifies arginine residues on histones H2A, H3, and H4 (H2 Arg3; H3 Arg2, H3 Arg8, H3 Arg17, H3 Arg26; and H4 Arg3). Several studies have suggested that PAD4-mediated citrullination has important implications for gene regulation. PAD4 may perform deimination at sites overlapping with arginine methylation, and antagonize the transcription-promoting effects of methylating enzymes such as CARM1 and PRMT1. PAD4 may therefore inhibit gene expression, most notably expression of cell-cycle regulating p53 genes such as p21. Previous studies have suggested that PAD4 may play central role in the development of cancer.

Immunogène

GST-tagged recombinant protein corresponding to human Protein-arginine deminiase 4.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Use Anti-Protein-arginine deiminase 4 Antibody, clone 10F1 (mouse monoclonal antibody) validated in WB, IP to detect PAD-4 also known as HL-60 PAD, Protein-arginine deiminase type-4, Peptidylarginine deiminase IV.

Qualité

Evaluated by Western Blot in DMSO treated and untreated HL-60 cell lysates.

Western Blot Analysis: 0.25 µg/mL of this antibody detected Protein-arginine deminiase 4 in 10 µg DMSO treated and untreated HL-60 cell lysates.

Description de la cible

~74 kDa observed

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
DMSO treated and untreated HL-60 cell lysates

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable