MABC87
Anti-CD148/DEP-1 Antibody, clone Ab1 (Azide Free)
clone Ab 1, from mouse
Synonyme(s) :
CD148, DEP-1, Receptor-type tyrosine-protein phosphatase eta, Protein-tyrosine phosphatase eta, R-PTP-eta, Density-enhanced phosphatase 1, DEP1, HPTP eta, Protein-tyrosine phosphatase receptor type J, R-PTP-J
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
Ab 1, monoclonal
Espèces réactives
human, mouse
Technique(s)
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
inhibition assay: suitable
western blot: suitable
Isotype
IgG1κ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... PTPRJ(5795)
Description générale
CD148, also known as Density-enhanced phosphatase 1 (DEP-1), is a membrane bound phosphatase that dephosphorylates a large number of protein targets and kinases thus serving as a regulator of many signaling cascades. CD148/DEP-1 plays a role in cell adhesion, migration, proliferation and differentiation. CD148/DEP-1 also plays a role in macrophage migration, adhesion and spreading. CD148/DEP-1 is also central to T-cell receptor signaling and acts as a negative regulator. CD148/DEP-1 acts as a positive regulator of endothelial cell survival and enhances the barrier function of epithelial junctions during reassembly. CD148/DEP-1 is also a key regulator of platelet activation and thrombosis as well as a tumor suppressor. CD148/DEP-1 is localized at the cell membrane particularly at cell projections, ruffle membranes, and cell junctions and it is expressed in lymphocytes and epithelial cells and expression increases as cell density increases.
Immunogène
His-tagged recombinant protein corresponding to Human CD148/DEP-1.
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-CD148/DEP-1 Antibody, clone Ab1 (Azide Free) is validated for use in Western Blotting and Immunohistochemistry (Paraffin) and Inhibition and Immunoprecipitation for the detection of CD148/DEP-1.
Western Blot Analysis: A representative lot of this antibody detected CD148/DEP-1 in Raji, HEK293 +/-CD148 HA transfection, and A431D +/- CD148 transfection cell lysates.
Western Blot Analysis: A representative lot of this antibody detected CD148/DEP-1 in Mouse kidney lysates (Takahashi, T., et al., (2006) Blood. 108:1234-1242).
Immunohistochemistry Analysis: A 1:50 dilution of a representative lot detected CD148/DEP-1 in Epithelial Cells of human prostate tissue.
Immunohistochemistry Analysis: A representative lot of this antibody detected CD148/DEP-1 in frozen mouse embryos. (Takahashi, T., et al., (2003) MOLECULAR AND CELLULAR BIOLOGY. 23(5):1817–1831).
Immunohistochemistry Analysis: A representative lot of this antibody detected CD148/DEP-1 in various mouse tissues such as kidney, cortex and cornea. (Takahashi, T., et al., (2006) Blood. 108:1234-1242).
Inhibition Assay: A representative lot of this antibody inhibited proliferation of HRMEC′s (Primary human renal microvascular endothelial cells) (Takahashi, T., et al., (2006) Blood. 108:1234-1242).
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated CD148/DEP-1 from Huvec and RMEC cell lysates (Takahashi, T., et al., (1999) J. Am. Soc. Nephrol. 10: 2135–2145).
Western Blot Analysis: A representative lot of this antibody detected CD148/DEP-1 in Mouse kidney lysates (Takahashi, T., et al., (2006) Blood. 108:1234-1242).
Immunohistochemistry Analysis: A 1:50 dilution of a representative lot detected CD148/DEP-1 in Epithelial Cells of human prostate tissue.
Immunohistochemistry Analysis: A representative lot of this antibody detected CD148/DEP-1 in frozen mouse embryos. (Takahashi, T., et al., (2003) MOLECULAR AND CELLULAR BIOLOGY. 23(5):1817–1831).
Immunohistochemistry Analysis: A representative lot of this antibody detected CD148/DEP-1 in various mouse tissues such as kidney, cortex and cornea. (Takahashi, T., et al., (2006) Blood. 108:1234-1242).
Inhibition Assay: A representative lot of this antibody inhibited proliferation of HRMEC′s (Primary human renal microvascular endothelial cells) (Takahashi, T., et al., (2006) Blood. 108:1234-1242).
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated CD148/DEP-1 from Huvec and RMEC cell lysates (Takahashi, T., et al., (1999) J. Am. Soc. Nephrol. 10: 2135–2145).
Qualité
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected CD148/DEP-1 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected CD148/DEP-1 in 10 µg of HeLa cell lysate.
Description de la cible
~260 kDa observed.
This protein is highly glycosylated and may run higher than the calculated molecular weight of 146 kDa (isoform 1) or 57 kDa (isoform 2). Uncharacterized bands may be observed in some lysates.
This protein is highly glycosylated and may run higher than the calculated molecular weight of 146 kDa (isoform 1) or 57 kDa (isoform 2). Uncharacterized bands may be observed in some lysates.
Forme physique
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Outil de sélection de produits.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
A monoclonal antibody against CD148, a receptor-like tyrosine phosphatase, inhibits endothelial-cell growth and angiogenesis.
Takahashi, Takamune, et al.
Blood, 108, 1234-1242 (2006)
Takamune Takahashi et al.
Molecular and cellular biology, 23(5), 1817-1831 (2003-02-18)
Vascularization defects in genetic recombinant mice have defined critical roles for a number of specific receptor tyrosine kinases. Here we evaluated whether an endothelium-expressed receptor tyrosine phosphatase, CD148 (DEP-1/PTPeta), participates in developmental vascularization. A mutant allele, CD148(DeltaCyGFP), was constructed to
T Takahashi et al.
Journal of the American Society of Nephrology : JASN, 10(10), 2135-2145 (1999-10-03)
Developmental assembly of the renal microvasculature requires spatially and temporally coordinated migration, assembly, differentiation, and maturation of endothelial cells in the context of adjacent epithelial and mesangial cells. In this study, endothelial expression and distribution of the receptor tyrosine phosphatase
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique