Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

MABC1187

Sigma-Aldrich

Anti-Apaf-1 Antibody, clone 18H2

clone 18H2, from rat

Synonyme(s) :

Apoptotic protease-activating factor 1

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rat

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

18H2, monoclonal

Espèces réactives

human, mouse

Technique(s)

western blot: suitable

Isotype

IgG2aκ

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

ambient

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... APAF1(317)
mouse ... Apaf1(11783)

Description générale

Apoptotic protease-activating factor 1 (UniProt: O88879; also known as Apaf-1) is encoded by the Apaf-1 gene (Gene ID: 11783) in murine species. Apaf-1 contains a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. It is highly expressed in lung and spleen, weakly in brain and kidney and is not detectable in liver. Under basal conditions it is a monomeric protein, but oligomerizes under apoptotic conditions. The monomeric form is auto-inhibited in a closed conformation through a bound ADP at the nucleotide binding site. Under apoptosis signals ADP is exchanged for ATP and its binding to cytochrome c triggers a conformation change where WD repeat region swings out, which allows the NB-ARC domain to rotate and expose the contact areas for oligomerization. Oligomeric form is a heptameric ring that binds to cytochrome c and ATP and is known as the apoptosome. Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 to mature caspase-9, which then activates effector caspases 3, 6, and 7.

Spécificité

Clone 18H2 detects Apaf-1 in human and mouse cells. It targets an epitope within the first 97 amino acids from the N-terminal region containing the CARD domain.

Immunogène

Epitope: N-terminus
Recombinant fragment corresponding to first 97 amino acids from the N-terminal region of murine Apaf-1.

Application

Anti-Apaf-1 Antibody, clone 18H2, Cat. No. MABC1187, is a rat monoclonal antibody that specifically detects Apaf-1. It has been tested for use in Western Blotting.
Research Category
Apoptosis & Cancer
Western Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Scott, C.L., et. al. (2004). J Cell Biol. 164(1):89-96).

Western Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Marsden, V.S., et. al. (2006). Blood. 107(5):1872-7).

Qualité

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Apaf-1 in 10 mg of SH-SY5Y cell lysate.




Description de la cible

~130 kDa observed;141 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified
Protein G purified
Purified rat monoclonal antibody IgG2a in PBS with 0.02% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

N V Srikanth Vallabani et al.
Mutagenesis, 34(3), 265-277 (2019-07-05)
Zinc oxide nanoparticles (ZnO NPs) with their wide range of consumer applications in day-to-day life received great attention to evaluate their effects in humans. This study has been attempted to elucidate the DNA damage response mechanism in a dermal model

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique