MABC1150
Anti-MAGEA3 Antibody, clone 57B
clone 57B, from mouse
Synonyme(s) :
Antigen MZ2-D, Cancer/testis antigen 1.3, CT1.3, MAGE-3 antigen
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.43
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
57B, monoclonal
Espèces réactives
human
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotype
IgG1κ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
ambient
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... MAGEA3(4102)
Description générale
Melanoma-associated antigen 3 (UniProt: P43357; also known as Antigen MZ2-D, Cancer/testis antigen 1.3, CT1.3, MAGE-3 antigen) is encoded by the MAGEA3 (also known as MAGE3) gene (Gene ID: 4102) in human. MAGE-3 is a member of the melanoma-associated antigen gene family of cancer antigens that are expressed in several neoplastic, but not normal, tissues with the exception of testes. In melanoma cells, it enhances viability and serves as an antigen for cytolytic T lymphocytes. MAGEA3 expression is not reported in kidney tumors, leukemias and lymphomas. MAGEA3 expression is normally limited to early developmental stages. It enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. It is also reported to enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. It is proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.
Spécificité
Clone 57B specifically targets a sequence in the N-terminal region of melanoma associated antigen 3 in human testes.
Immunogène
Epitope: domain of:
His-tagged recombinant human melanoma associated antigen 3 containing the polyhistidine tail.
Application
Anti-MAGEA3 Antibody, clone 57B, Cat. No. MABC1150, detects multiple MAGE-A proteins. Validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunocytochemistry Analysis: A representative lot detected MAGEA3 in Immunocytochemistry applications. (Hofbauer, G.F., et. al. (1997) Am J Pathol. 151(6):1549-53; Landry, C., et. al. (2000). Int J Cancer. 86(6):835-41; Kocher, T., et. al. (1995). Cancer Res. 55(11):2236-9; Juretic, A., et. al. (2003). Lancet Oncol. 4(2):104-9).
Western Blotting Analysis: A representative lot detected MAGEA3 in Western Blot applications. (Kocher, T., et. al. (1995). Cancer Res. 55(11):2236-9; Rimoldi, D., et. al. (2000). Int J Cancer. 86(5):749-51; SHultz-Thater, E., et. al. (2011). Int J Cancer. 129(5):1137-48).
Immunohistochemistry Analysis: A representative lot detected MAGEA3 in Immunohistochemistry applications. (Hofbauer, G.F., et. al. (1997) Am J Pathol. 151(6):1549-53; Aubry, F., et. al. (2001). Cancer. 92(11):2778-85; Cheville, J.C., et. al. (1999). Mod Pathol. 12(10):974-8; Chitale D.A., et. al. (2005). Mod Pathol. 18(1):119-26; Groeper, C., et. al. (2007). Int J Cancer. 120(2):337-43; Juretic, A., et. al. (2003). Lancet Oncol. 4(2):104-9.
Western Blotting Analysis: A representative lot detected MAGEA3 in Western Blot applications. (Kocher, T., et. al. (1995). Cancer Res. 55(11):2236-9; Rimoldi, D., et. al. (2000). Int J Cancer. 86(5):749-51; SHultz-Thater, E., et. al. (2011). Int J Cancer. 129(5):1137-48).
Immunohistochemistry Analysis: A representative lot detected MAGEA3 in Immunohistochemistry applications. (Hofbauer, G.F., et. al. (1997) Am J Pathol. 151(6):1549-53; Aubry, F., et. al. (2001). Cancer. 92(11):2778-85; Cheville, J.C., et. al. (1999). Mod Pathol. 12(10):974-8; Chitale D.A., et. al. (2005). Mod Pathol. 18(1):119-26; Groeper, C., et. al. (2007). Int J Cancer. 120(2):337-43; Juretic, A., et. al. (2003). Lancet Oncol. 4(2):104-9.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Qualité
Evaluated by Immunohistochemistry in human testis tissue.
Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected MAGEA3 in human testis tissue.
Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected MAGEA3 in human testis tissue.
Description de la cible
34.75 kDa calculated.
Forme physique
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
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Adoptive cancer immunotherapy using DNA-demethylated T helper cells as antigen-presenting cells.
Kirkin AF, Dzhandzhugazyan KN, Guldberg P, Fang JJ, Andersen RS, Dahl C, Mortensen J et al.
Nature Communications, 9(1), 785-785 null
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