MAB4145
Anti-BCRP Antibody, clone BXP-34
culture supernatant, clone BXP-34, Chemicon®
Synonyme(s) :
Breast Cancer Resistance Protein, Placenta-specific ATP Binding Cassette Transporter, ATP-binding Cassette, Sub Family G, member 2 (ABCG2)
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.43
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
culture supernatant
Type de produit anticorps
primary antibodies
Clone
BXP-34, monoclonal
Espèces réactives
human
Fabricant/nom de marque
Chemicon®
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
Entrée
sample type hematopoietic stem cell(s)
sample type neural stem cell(s)
Isotype
IgG1
Adéquation
not suitable for Western blot
not suitable for immunohistochemistry (Paraffin)
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... ABCG2(9429)
Description générale
The 72 kDa breast cancer resistance protein (BCRP) is the second member of the subfamily G of the human ATP binding cassette (ABC) transporter superfamily and thus also designated as ABCG2. BCRP is a half-transporter consisting of only 1 nucleotide binding domain followed by 1 membrane-spanning domain. Current experimental evidence suggests that BCRP may function as a homodimer or homotetramer. Overexpression of BCRP is associated with high levels of resistance to a variety of anticancer agents. BCRP expression has been detected in a large number of hematological malignancies and solid tumors, indicating that this transporter may play an important role in clinical drug resistance of cancers. In addition to its role to confer resistance against chemotherapeutic agents, BCRP actively transports structurally diverse organic molecules, conjugated or unconjugated, such as estrone-3-sulfate, 17-β-estradiol 17-(β-D-glucuronide), and methotrexate. BCRP is highly expressed in the placental syncytiotrophoblasts, in the apical membrane of the epithelium in the small intestine, in the liver canalicular membrane, and at the luminal surface of the endothelial cells of human brain microvessels. This strategic and substantial tissue localization indicates that BCRP also plays an important role in absorption, distribution, and elimination of drugs that are BCRP substrates.
Spécificité
This antibody reacts with an internal epitope of the Breast Cancer Resistance Protein (BCRP), a 70 kDa transmembrane half-transporter which is involved in multidrug resistance. The antibody does not cross-react with human MDR1, MRP1, MRP2 or MRP5 gene products.
Immunogène
The Mitoxanthrone resistant, BCRP-overexpressing cell line MCF7-MR.
Application
Detect BCRP using this Anti-BCRP Antibody, clone BXP-34 validated for use in IC, IH.
Immunocytochemistry: 1:20 - 1:50 on acetone fixed cytospin preparations
Immunohistochemistry: 1:20 on acetone fixed frozen tissue sections
Not suitable for paraffin-embedded tissues or for Western blotting
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 1:20 on acetone fixed frozen tissue sections
Not suitable for paraffin-embedded tissues or for Western blotting
Optimal working dilutions must be determined by end user.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Hematopoietic Stem Cells
Hematopoietic Stem Cells
Description de la cible
70 kDa
Forme physique
Liquid containing 0.7% BSA and 0.1% sodium azide.
Stockage et stabilité
Maintain refrigerated at 2-8°C for up to 6 months. For long-term storage store at -20°C.
Remarque sur l'analyse
Control
MCF-7 cells
MCF-7 cells
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
En option
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Wen Jing et al.
Oncology letters, 20(6), 309-309 (2020-10-24)
Most patients with advanced leukemia eventually die from multidrug resistance (MDR). Chemotherapy-resistant leukemia cells may lead to treatment failure and disease relapse. Overexpression of ATP-binding cassette subfamily G member 2 (ABCG2) leads to MDR, which serves as a potential biomarker
Maria Paprocka et al.
European journal of haematology, 99(5), 415-422 (2017-08-15)
It is well established that expression of multi-drug resistance (MDR) proteins (MDR1, BCRP, MDR3, MRP1, and LRP) in leukemic blasts correlates with acute myeloid leukemia (AML) patients' clinical response. Assuming that leukemic stem cells (LSC) are resistant to chemotherapy and
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique