MAB388
Anti-Myelin Proteolipid Protein Antibody, CT, clone PLPC1
clone PLPC1, Chemicon®, from mouse
Synonyme(s) :
PLP
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
PLPC1, monoclonal
Réactivité de l'espèce (prédite par homologie)
mammals
Fabricant/nom de marque
Chemicon®
Technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
Isotype
IgG2a
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... PLP1(5354)
Spécificité
Reacts with myelin proteolipid protein in many mammalian species. Shows cross-reactivity to DM20.
Immunogène
Epitope: C-terminus
Myelin proteolipid protein, synthetic peptide CGRGTKF corresponding to the C-terminal peptide.
Application
Detect Myelin Proteolipid Protein using this Anti-Myelin Proteolipid Protein Antibody, C-terminus, clone PLPC1 validated for use in IH, IH(P) & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neurochemistry & Neurotrophins
Neuronal & Glial Markers
Neurochemistry & Neurotrophins
Western Blotting: detects a 23-25kDa protein and DM20 a 20kDa protein; PLP can aggregate causing HMW bands to sometimes appear in certain preparations.
Immunocytochemistry: 4% PFA fixed cells, 5-10 minutes room temperature. Use 0.1% triton in block only to allow antibody access to C-terminal epitope.
Immunohistology on fresh frozen sections or 4% PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2% triton X-100 in the blocking step for permeabilization.
Immunohistochemistry in paraffin: works best with lightly fixed tissues, or Bouin′s fixed material, although there are reports of traditional formalin fixation also working with antigen recovery. {Mathis, et al 2003}.
Optimal working dilutions must be determined by end user.
Immunocytochemistry: 4% PFA fixed cells, 5-10 minutes room temperature. Use 0.1% triton in block only to allow antibody access to C-terminal epitope.
Immunohistology on fresh frozen sections or 4% PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2% triton X-100 in the blocking step for permeabilization.
Immunohistochemistry in paraffin: works best with lightly fixed tissues, or Bouin′s fixed material, although there are reports of traditional formalin fixation also working with antigen recovery. {Mathis, et al 2003}.
Optimal working dilutions must be determined by end user.
Description de la cible
23-25 kDa
Liaison
Replaces: MAB388
Forme physique
Format: Purified
Protein A purified
Purified mouse immunoglobulin in PBS, pH 7.2 containing 0.09% sodium azide.
Stockage et stabilité
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Remarque sur l'analyse
Control
Brain
Brain
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Sugimoto, Y, et al.
Development, 128, 3321-3330 (2001)
D W Bayless et al.
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C M Cheng et al.
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