MAB3582
Anti-Fascin Antibody, clone 55K2
clone 55K2, Chemicon®, from mouse
Synonyme(s) :
Anti-FAN1, Anti-SNL, Anti-p55
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
55K2, monoclonal
Espèces réactives
echinoderm, sea urchin, gerbil, human
Réactivité de l'espèce (prédite par homologie)
mammals
Fabricant/nom de marque
Chemicon®
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotype
IgG1
Adéquation
not suitable for immunoprecipitation
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... FSCN1(6624)
Catégories apparentées
Spécificité
Fascin. The antibody is strongly positive when antigen-presenting dendritic cells are matured. MAB3582 is a sensitive marker for Reed-Sternberg Cells of Hodgkin′s Disease. The protein is an actin bundling protein specifically localized in filopodia and growth cones.
Immunogène
Human fascin
Application
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
This Anti-Fascin Antibody, clone 55K2 is validated for use in WB, IC, IH(P) for the detection of Fascin.
Western blot. Recognizes a protein of 55 kDa. Suggested working dilution is 1:100-1:1,000. Suggested blocking buffer is 3% BSA in PBS or 3% non fat milk in PBS.
Immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Suggested working dilution is 1:100-1:1,000.
Immunocytochemistry on cold methanol fixed cultured cells. Suggested working dilution is 1:100-1:1,000.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Suggested working dilution is 1:100-1:1,000.
Immunocytochemistry on cold methanol fixed cultured cells. Suggested working dilution is 1:100-1:1,000.
Optimal working dilutions must be determined by the end user.
Forme physique
Format: Purified
Liquid in 0.02M Phosphate buffer with 0.25M NaCl and 0.1% sodium azide.
Stockage et stabilité
Maintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt.
Remarque sur l'analyse
Control
POSITIVE CONTROL:
Hela cells, neuronal tissues, antigen presenting dendritic cells.
POSITIVE CONTROL:
Hela cells, neuronal tissues, antigen presenting dendritic cells.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
S Al-Saad et al.
British journal of cancer, 99(9), 1476-1483 (2008-10-16)
Vimentin, nuclear factor-kappaB (NF-kappaB) p105, fascin, E-cadherin, TGF-beta, Par6 and atypical PKC are molecular markers that play an important role in cell differentiation. Herein, we investigate their prognostic impact in primary non-small-cell carcinoma (NSCLC). Tumour tissue samples from 335 resected
The prognostic impact of TGF-?1, fascin, NF-?B and PKC-? expression in soft tissue sarcomas.
Valkov, A; Sorbye, SW; Kilvaer, TK; Donnem, T; Smeland, E; Bremnes, RM; Busund, LT
Testing null
Protein expression dynamics during postnatal mouse brain development.
Laeremans, A; Van de Plas, B; Clerens, S; Van den Bergh, G; Arckens, L; Hu, TT
Journal of experimental neuroscience null
C S Cohan et al.
Cell motility and the cytoskeleton, 48(2), 109-120 (2001-02-13)
Growth cones at the distal tips of growing nerve axons contain bundles of actin filaments distributed throughout the lamellipodium and that project into filopodia. The regulation of actin bundling by specific actin binding proteins is likely to play an important
S Yamashiro-Matsumura et al.
The Journal of cell biology, 103(2), 631-640 (1986-08-01)
The 55-kD protein is a new actin-bundling protein purified from HeLa cells (Yamashiro-Matsumura, S., and F. Matsumura, 1985, J. Biol. Chem., 260:5087-5097). We have prepared monoclonal antibodies against the 55-kD protein and examined its intracellular localization, as well as its
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