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Key Documents

MAB331

Sigma-Aldrich

Anti-SNAP-25 Antibody, clone SP14

ascites fluid, clone SP14, Chemicon®

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Forme d'anticorps

ascites fluid

Clone

SP14, monoclonal

Espèces réactives

pig, human, hamster, rat

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

Isotype

IgG1

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... SNAP25(6616)

Spécificité

SNAP-25 (26-27 kDa). MAB331 is reactive with fusion protein from COS cells, but not with fusion protein from bacterial systems.

Immunogène

Crude synaptic immunoprecipitate (Human)

Application

Detect SNAP-25 using this Anti-SNAP-25 Antibody, clone SP14 validated for use in ELISA, WB, IH(P).
Immunohistochemistry at 1:500-1:5,000

Formalin fixed (<4 weeks), paraffin embedded

* It is suggested that you use an IgG specific second antibody to minimize possible non-specific staining.

Frozen

Western blotting at 1:100-1:1,000

ELISA at 1:500

It is reported that clone SP14 is capable of detecting SDS-resistant SNARE complexes {J. Cell Sci 2004 117(6):955-966}.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Synapse & Synaptic Biology

Forme physique

Liquid. Contains no preservative.

Stockage et stabilité

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Christian Schulz,Benjamin Stoelcker,Brigitte Ruhland,Nico Jentsch,Andreas Steege
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Matthew J Van Hook et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(17), 4618-4634 (2017-04-02)
G-protein βγ subunits (Gβγ) interact with presynaptic proteins and regulate neurotransmitter release downstream of Ca2+ influx. To accomplish their roles in sensory signaling, photoreceptor synapses use specialized presynaptic proteins that support neurotransmission at active zone structures known as ribbons. While
High sensitivity of mouse neuronal cells to tetanus toxin requires a GPI-anchored protein.
Munro, P; Kojima, H; Dupont, JL; Bossu, JL; Poulain, B; Boquet, P
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The gene for soluble N-ethylmaleimide sensitive factor attachment protein alpha is mutated in hydrocephaly with hop gait (hyh) mice.
Hong, HK; Chakravarti, A; Takahashi, JS
Proceedings of the National Academy of Sciences of the USA null
Most peptide-containing sensory neurons lack proteins for exocytotic release and vesicular transport of glutamate.
Morris, JL; Konig, P; Shimizu, T; Jobling, P; Gibbins, IL
The Journal of Comparative Neurology null

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