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Key Documents

MAB2510

Sigma-Aldrich

Anti-Green Fluorescent Protein Antibody, original GFP only, clone 264-449-2

clone 264-449-2, Chemicon®, from mouse

Synonyme(s) :

GFP

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

264-449-2, monoclonal

Réactivité de l'espèce (prédite par homologie)

all

Conditionnement

antibody small pack of 25 μg

Fabricant/nom de marque

Chemicon®

Technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG1

Numéro d'accès UniProt

Conditions d'expédition

ambient

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Description générale

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Spécificité

Directed against a bacterially-expressed fusion protein. Also reacts with red-shifted GFP-variant. Does not react with enhanced GFP.

Immunogène

Epitope: original GFP only
highly-purified native GFP

Application

Anti-Green Fluorescent Protein Antibody, original GFP only, clone 264-449-2 is an antibody against Green Fluorescent Protein for use in IP, WB & IC.
Immunocytochemistry

Immunoblotting: 1:1000

Immunoprecipitation

Optimal working dilutions and protocols must be determined by the end user.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Qualité

Tested

Forme physique

Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.

Stockage et stabilité

Store at 2-8°C for up to 12 months.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

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Maria A Carmona, Keith K Murai, Lei Wang, Amanda J Roberts, Elena B Pasquale et al.
Proceedings of the National Academy of Sciences of the USA null
R Rauchenberger et al.
Current biology : CB, 7(3), 215-218 (1997-03-01)
Cells of the unicellular eukaryote Dictyostelium discoideum take up all their nutrients by endocytosis. Both particle- and fluid-containing vacuoles are transiently surrounded by a cytoskeletal coat [1] [2]. When this coat has dissociated, acidification and digestion of the vesicle contents
Jack Phan et al.
Human molecular genetics, 18(6), 1006-1016 (2009-01-07)
In addition to the hallmark neurological manifestations of Huntington's disease (HD), weight loss with metabolic dysfunction is often observed in the later stages of disease progression and is associated with poor prognosis. The mechanism for weight loss in HD is
Modulation of integrin-linked kinase nucleo-cytoplasmic shuttling by ILKAP and CRM1.
Kerry-Ann Nakrieko,Alisa Vespa,David Mason,Timothy S Irvine,Sudhir J A D'Souza,Lina Dagnino
Cell Cycle null
Fei Mao et al.
Acta neuropathologica, 142(4), 629-642 (2021-06-22)
The neurodegenerative diseases amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration with TAR DNA-binding protein-43 (TDP-43) inclusions (FTLD-TDP) share the neuropathological hallmark of aggregates of TDP-43. However, factors governing the severity and regional distribution of TDP-43 pathology, which may account

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