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Key Documents

MAB1222A

Sigma-Aldrich

Anti-Tryptase Antibody, Mast Cell, clone G3, Alkaline Phosphatase

clone G3, Chemicon®, from mouse

Synonyme(s) :

Anti-TPS1, Anti-TPSB1, Anti-TPSB2, Anti-Tryptase-2

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Conjugué

alkaline phosphatase conjugate

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

G3, monoclonal

Espèces réactives

human

Fabricant/nom de marque

Chemicon®

Technique(s)

immunohistochemistry: suitable
western blot: suitable

Isotype

IgG1κ

Numéro d'accès NCBI

NM_003294.3

Numéro d'accès UniProt

Q15661

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TPSAB1(7177)

Spécificité

Mast cell tryptase. Will show reactivity to basophils but to a lesser degree.

Application

Anti-Tryptase Antibody, Mast Cell, clone G3, Alkaline Phosphatase is an antibody against Tryptase for use in WB, IH.
Immunoblotting & immunohistochemistry at 1:200-1:1,000. For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue. May be used on thin sections prepared for ultrastructural analysis after fixation of tissue in a modified Karnovsky′s reagent [Lab. Invest. (1988). 58:682-691].

Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms

Forme physique

In 0.05M Tris + 0.1M NaCl + 0.001M MgCl2 with 0.1% NaN3, pH=8.0.

Stockage et stabilité

Maintain at +2-8°C in undiluted aliquots for up to 6 months.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Novel site-specific mast cell subpopulations in the human lung.
C K Andersson, M Mori, L Bjermer, C-G Lofdahl, J S Erjefalt, C K Andersson, M Mori et al.
Thorax null
Characterization of mast-cell tryptase-expressing peripheral blood cells as basophils
Foster, B. et al.
The Journal of Allergy and Clinical Immunology, 109, 287-293 (2002)
S Ying et al.
American journal of respiratory cell and molecular biology, 9(4), 356-360 (1993-10-01)
We have investigated the phenotype of interleukin-5 (IL-5) mRNA+ cells in the nasal mucosa of subjects with allergic rhinitis. Serial cryostat sections were cut from paraformaldehyde-fixed snap-frozen nasal biopsies from six patients, before and 24 h after local allergen provocation
W R Sperr et al.
Blood, 98(7), 2200-2209 (2001-09-25)
alpha- and beta-tryptase genes encode serine proteases that are abundantly expressed by mast cells. Under physiologic conditions other myeloid cells are virtually tryptase negative. However, tryptases are also expressed in several myeloid leukemia cell lines. In this study, serum total
Premkumar Siddhuraj et al.
Frontiers in immunology, 13, 924244-924244 (2022-08-20)
The mast cell-specific metalloprotease CPA3 has been given important roles in lung tissue homeostasis and disease pathogenesis. However, the dynamics and spatial distribution of mast cell CPA3 expression in lung diseases remain unknown. Using a histology-based approach for quantitative spatial
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