MAB1222A
Anti-Tryptase Antibody, Mast Cell, clone G3, Alkaline Phosphatase
clone G3, Chemicon®, from mouse
Synonyme(s) :
Anti-TPS1, Anti-TPSB1, Anti-TPSB2, Anti-Tryptase-2
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
alkaline phosphatase conjugate
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
G3, monoclonal
Espèces réactives
human
Fabricant/nom de marque
Chemicon®
Technique(s)
immunohistochemistry: suitable
western blot: suitable
Isotype
IgG1κ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... TPSAB1(7177)
Spécificité
Mast cell tryptase. Will show reactivity to basophils but to a lesser degree.
Application
Anti-Tryptase Antibody, Mast Cell, clone G3, Alkaline Phosphatase is an antibody against Tryptase for use in WB, IH.
Immunoblotting & immunohistochemistry at 1:200-1:1,000. For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue. May be used on thin sections prepared for ultrastructural analysis after fixation of tissue in a modified Karnovsky′s reagent [Lab. Invest. (1988). 58:682-691].
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
Inflammation & Autoimmune Mechanisms
Forme physique
In 0.05M Tris + 0.1M NaCl + 0.001M MgCl2 with 0.1% NaN3, pH=8.0.
Stockage et stabilité
Maintain at +2-8°C in undiluted aliquots for up to 6 months.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Novel site-specific mast cell subpopulations in the human lung.
C K Andersson, M Mori, L Bjermer, C-G Lofdahl, J S Erjefalt, C K Andersson, M Mori et al.
Thorax null
Characterization of mast-cell tryptase-expressing peripheral blood cells as basophils
Foster, B. et al.
The Journal of Allergy and Clinical Immunology, 109, 287-293 (2002)
S Ying et al.
American journal of respiratory cell and molecular biology, 9(4), 356-360 (1993-10-01)
We have investigated the phenotype of interleukin-5 (IL-5) mRNA+ cells in the nasal mucosa of subjects with allergic rhinitis. Serial cryostat sections were cut from paraformaldehyde-fixed snap-frozen nasal biopsies from six patients, before and 24 h after local allergen provocation
W R Sperr et al.
Blood, 98(7), 2200-2209 (2001-09-25)
alpha- and beta-tryptase genes encode serine proteases that are abundantly expressed by mast cells. Under physiologic conditions other myeloid cells are virtually tryptase negative. However, tryptases are also expressed in several myeloid leukemia cell lines. In this study, serum total
Premkumar Siddhuraj et al.
Frontiers in immunology, 13, 924244-924244 (2022-08-20)
The mast cell-specific metalloprotease CPA3 has been given important roles in lung tissue homeostasis and disease pathogenesis. However, the dynamics and spatial distribution of mast cell CPA3 expression in lung diseases remain unknown. Using a histology-based approach for quantitative spatial
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