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Key Documents

HTS100RTA

Sigma-Aldrich

Ready-to-Assay delta Opioid Receptor Frozen Cells

Human

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About This Item

Code UNSPSC :
41106514
eCl@ss :
32011203
Nomenclature NACRES :
NA.81

product name

Ready-to-Assay delta Opioid Receptor Frozen Cells, Human Delta / OP1 / DOP / DOR GPCR frozen cells for Calcium Flux FLIPR Assays.

Source biologique

human

Niveau de qualité

100

Fabricant/nom de marque

Ready-to-Assay

Technique(s)

calcium flux assay: suitable

Numéro d'accès NCBI

NM_000911

Méthode de détection

fluorometric

Conditions d'expédition

dry ice

Description générale

Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Opiates derived from the opium poppy, Papaver somniferum, have been used in for millenia for their anti-diarrheal, analgesic and euphoric properties. More recently, endogenous peptides, enkephalins, dynorphins, and endorphins, were found to bind to the same sites as opiate alkaloids. The receptors for the classical opioids are three related GPCRs, δ, κ, andμ also known as OP1, OP2 and OP3, respectively), that activate Gi/o to reduce intracellular cAMP levels. Although most clinically used opioids function by activation of the μ opioid receptor, agonist of spinal δ opioid receptors have antinociceptive activity that is independent of μ. In addition, activation of δ increases locomotor activity, inhibits gastrointestinal motility, and decreases respiratory frequency (Dhawan et al., 1996). Agonists for δ opioid receptors also exhibit antidepressant-like activity in animal models (Broom et al., 2002). Millipore’s cloned human δ opioid -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant δ opioid expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at δ.

Description de la lignée cellulaire

GPCR Cell Lines
Host cells: Chem-1

Actions biochimiques/physiologiques

GPCR Class: A
Protein Target: Delta / OP1 / DOP / DOR
Target Sub-Family: Opioid

Composants

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.

Fifty (50) mL of Media Component.

Autres remarques

Limited to a single use and shall not be propagated and/or re-frozen by licensee.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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