FC010

Fibronectine plasmatique humaine, protéine purifiée

This Human plasma fibronectin is a purified protein, used as an attachment factor suitable for cell propagation in vitro.

• Code UNSPSC : 12352202
• eCl@ss : 32160405
• Nomenclature NACRES : NA.75

Propriétés

• Source biologique: human
• Niveau de qualité: 100; 300
• Stérilité: sterile; sterile-filtered
• Pureté: ~95% (SDS-PAGE)
• Forme: liquid
• Poids mol.: 220 kDa
• Fabricant/nom de marque: Chemicon^®
• Concentration: 1 mg/mL
• Technique(s): cell culture | mammalian: suitable
• Couverture de surface: 2—10 μg/cm²
• Entrée: sample type mesenchymal stem cell(s); sample type neural stem cell(s); sample type: human embryonic stem cell(s); sample type epithelial cells; sample type hematopoietic stem cell(s); sample type induced pluripotent stem cell(s); sample type pancreatic stem cell(s)
• Numéro d'accès NCBI: NM_002026.2; NM_054034.2; NM_212474.1; NM_212475.1; NM_212476.1; NM_212478.1; NM_212482.1
• Numéro d'accès UniProt: P02751
• Spécificité de la liaison: Peptide Source: Collagen; ; Peptide Source: Laminin
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Informations sur le gène: human ... FN1(2335)

Description

Description générale

Fibronectins (FNs) are high-molecular-mass adhesive glycoproteins of the extracellular matrix (ECM) and body fluids. It is a dimer composed of two subunits linked by two disulfide bonds. Each monomer of FN consists of three types of repeating units: 12 FN repeats of type I (approximately 40 amino acids each), two type II repeats (around 60 amino acids), and 15-17 type III repeats (approximately 90 amino acids). These repeating units contribute to the structural and functional diversity of fibronectin molecules.

Application

Human fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads.

Actions biochimiques/physiologiques

Fibronectin plays a crucial role in mediating diverse cellular interactions and is involved in various processes, including cell adhesion, the establishment and maintenance of cell morphology, cell migration, growth, and differentiation. It interacts with several extracellular matrix (ECM) and cell surface proteins, such as collagen, heparin, fibrin, and specific cell membrane receptors. Fibronectin can also serve as a ligand for multiple integrins, including the well-known fibronectin receptor alpha5-beta1, which binds to the arginylglycylaspartic acid (RGD) sequence in the repeat III-10 region of fibronectin. This extensive network of interactions highlights fibronectin′s significance in regulating cellular behavior and mediating tissue function.

Forme physique

Forme liquide dans du NaCl 150 mM et du phosphate de sodium 10 mM, pH 7,5, stérilisée par filtration, ne contenant aucun conservateur.

Stockage et stabilité

Conserver entre 2 et 8 °C jusqu′à 6 mois après la date de réception. Ne pas congeler.

Remarque sur l'analyse

Une double bande de 220 kDa apparaît en conditions de réduction. La FNh est purifiée par chromatographie d′affinité sur gélatine-agarose suivie d′une chromatographie sur héparine-agarose. Un dépistage a été réalisé sur le plasma de donneurs qui s′est révélé négatif pour le VIH, le HTLV, l′hépatite B et l′hépatite C.

Autres remarques

Suggested Procedure for Coating Cell Cultureware

1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm²) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm².

2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.

3.Introduce the proper CO2 atmosphere, if required.

4.Add the calculated amount of HFN to each culture vessel.

5.Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.

6.Remove residual balanced salt solution before proceeding with standard cell culture procedures

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Sauf indication contraire dans notre catalogue ou tout autre document associé au(x) produit(s) concerné(s), nos produits sont uniquement destinés aux activités de recherche et ne sauraient être utilisés à d'autres fins, ce qui inclut, sans toutefois s'y limiter, les utilisations commerciales non autorisées, les utilisations diagnostiques in vitro, les utilisations thérapeutiques ex vivo ou in vivo ou tout type de consommation ou d'application chez l'homme ou l'animal.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3