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CBL154

Sigma-Aldrich

Anti-H-CAM Antibody, clone F10-44-2

clone F10-44-2, Chemicon®, from mouse

Synonyme(s) :

CD44

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

F10-44-2, monoclonal

Espèces réactives

human

Fabricant/nom de marque

Chemicon®

Technique(s)

flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable

Isotype

IgG2a

Numéro d'accès NCBI

NM_000610.3
NM_001001389.1
NM_001001390.1
NM_001001391.1
NM_001001392.1

Numéro d'accès UniProt

P16070

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CD44(960)

Catégories apparentées

Application

Identification of CD44 positive cells by flow cytometry and immunocytochemistry, in particular with formalin fixed paraffin wax embedded sections of lymphoid and epithelial tissues (high temperature antigen retrieval in 10mM citrate buffer, pH6.0, is recommended).

Studies involving T, B cell, monocyte, granulocyte T and B cell binding to HEV during lymphocyte circulation and movement of leucocytes to inflammatory sites.

Studies on HCAM (homing receptor) function.

Immunoprecipitation.

Optimal working dilutions must be determined by the end user.
This Anti-H-CAM Antibody, clone F10-44-2 is validated for use in FC, IP, IH, IH(P) for the detection of H-CAM.

Forme physique

Format: Purified

Remarque sur l'analyse

Control
POSITIVE CONTROL: Tonsil; stains all T-cells in the paracortex

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Biochemical characterisation and localization in brain of a human brain-leucocyte membrane glycoprotein recognised by a monoclonal antibody.
McKenzie, J L, et al.
Journal of Neurochemistry, 39, 1461-1466 (1982)
Identification of mRNA that encodes an alternative form of H-CAM(CD44) in lymphoid and nonlymphoid tissues.
Goldstein, L A and Butcher, E C
Immunogenetics, 32, 389-397 (1990)
José M Díez et al.
Stem cell research & therapy, 6, 28-28 (2015-04-19)
Fetal bovine serum (FBS) is an animal product used as a medium supplement. The animal origin of FBS is a concern if cultured stem cells are to be utilized for human cell therapy. Therefore, a substitute for FBS is desirable.
Arantxa Blázquez-Prunera et al.
Stem cell research & therapy, 8(1), 103-103 (2017-04-30)
Mesenchymal stem cells (MSCs) show promising characteristics for their use in advanced therapy medicinal products. However, there are some unresolved concerns, such as the use of animal components for their expansion. In this study we assessed the suitability of a

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