ABS1024
Anti-Angiomotin Antibody
serum, from rabbit
Synonyme(s) :
Angiomotin
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
serum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
human, rat, mouse
Technique(s)
immunocytochemistry: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... AMOT(154796)
Description générale
Angiomotin is a cell surface protein that plays a central role in tight junction maintenance and the uptake of proteins at tight junctions but also appears to regulate endothelial cell response and their migration toward growth factors as well as their response to angiostatin. Thus Angiomotin plays a role in blood vessel formation, cell migration, protein localization, chemotaxis, cellular polarity and many more cell-movement/migration activities. Angiomotin is a component of a multi-protein complex associated at tight junctions that includes ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3 proteins. Angiomotin is expressed in endothelial cells and found in placenta, blood vessels and skeletal muscle tissue, as well as at the leading edge of lamellipodia and cellular processes.
Immunogène
Epitope: C-terminus
Recombinant protein corresponding to the C-terminus of human Angiomotin.
Application
Research Category
Signaling
Signaling
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
This Anti-Angiomotin Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of Angiomotin.
Western Blotting Analysis: A representative lot detected Angiomotin in MDCK cell lysate (Wells, C. D., et al. (2006). Cell. 125:535-548).
Immunocytochemistry Analysis: A representative lot detected Angiomotin in MDCK cells (Wells, C. D., et al. (2006). Cell. 125:535-548).
Immunocytochemistry Analysis: A representative lot detected Angiomotin in MDCK cells (Wells, C. D., et al. (2006). Cell. 125:535-548).
Qualité
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Angiomotin in 10 µg of HEK293 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Angiomotin in 10 µg of HEK293 cell lysate.
Description de la cible
~150/80 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing at ~118 kDa and ~73 kDa This high molecular weight observed could be due to high phosphorylation.
Forme physique
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Clark D Wells et al.
Cell, 125(3), 535-548 (2006-05-09)
Using functional and proteomic screens of proteins that regulate the Cdc42 GTPase, we have identified a network of protein interactions that center around the Cdc42 RhoGAP Rich1 and organize apical polarity in MDCK epithelial cells. Rich1 binds the scaffolding protein
Ziying Han et al.
The Journal of biological chemistry, 295(25), 8596-8601 (2020-05-10)
The Ebola virus (EBOV) VP40 matrix protein (eVP40) orchestrates assembly and budding of virions in part by hijacking select WW-domain-bearing host proteins via its PPxY late (L)-domain motif. Angiomotin (Amot) is a multifunctional PPxY-containing adaptor protein that regulates angiogenesis, actin
Jacob J Adler et al.
The Journal of biological chemistry, 288(21), 15181-15193 (2013-04-09)
The adaptor protein Amot130 scaffolds components of the Hippo pathway to promote the inhibition of cell growth. This study describes how Amot130 through binding and activating the ubiquitin ligase AIP4/Itch achieves these effects. AIP4 is found to bind and ubiquitinate
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