ABN1474

Anti-RNF213

from rabbit, purified by affinity chromatography

• Synonyme(s) : E3 ubiquitin-protein ligase RNF213, EC:2.3.2.27, ALK lymphoma oligomerization partner on chromosome 17, Mysterin, RING finger protein 213, RING-type E3 ubiquitin transferase RNF213
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μg
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NP_001243000
• Numéro d'accès UniProt: Q63HN8
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... RNF213(57674)

Description

Description générale

E3 ubiquitin-protein ligase RNF213 (UniProt: Q63HN8; also known as EC:2.3.2.27, ALK lymphoma oligomerization partner on chromosome 17, Mysterin, RING finger protein 213, RING-type E3 ubiquitin transferase RNF213) is encoded by the RNF213 (also known as ALO17, C17orf27, KIAA1554, KIAA1618, MYSTR) gene (Gene ID: 57674) in human. RNF213 is a widely expressed, homooligomeric cytosolic protein that is involved in the non-canonical Wnt signaling pathway in vascular development. It acts by mediating ubiquitination and degradation of FLNA and NFATC2 downstream of RSPO3, which leads to the inhibition of the non-canonical Wnt signaling pathway and promotes vessel regression. Its activity is induced by pro-inflammatory cytokines. RNF213 contains a Zinc finger domain (aa 3997-4036), which is required for its ubiquitin-protein ligase activity. It also contains an AAA domain, which is associated with ATPase activity. Four isoforms of RNF213 have been described that are produced by alternative splicing. Mutations in RNF213 gene have been linked to Moyamoya disease 2 (MYMY2) disease, a progressive cerebral angiopathy that can lead to transient ischemic attacks and/or cerebral infarction, and rupture of the collateral vessels can cause intracranial hemorrhage.

Spécificité

This rabbit polyclonal antibody detects E3 ubiquitin-protein ligase RNF213 in human cells. It targets an epitope within 15 amino acids from the C-terminal half.

Immunogène

Epitope: unknown

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal half of human E3 ubiquitin-protein ligase RNF213. Immunogen sequence is conserved in isoforms 1, 2, and 4.

Application

Anti-RNF213, Cat. No. ABN1474, is a highly specific rabbit polyclonal antibody that targets E3 ubiquitin-protein ligase RNF213 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected RNF213 in human cerebral cortex and human kidney tissue sections.

Western Blotting Analysis: 2 µg/mL from a representative lot detected RNF213 in 10 µg of HEK293 cells transfected with 3XFLAG RNF213 WT.

Research Category
Neuroscience

Qualité

Evaluated by Western Blotting in HUVEC lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected RNF213 in 10 µg of HUVEC lysate.

Description de la cible

~590 kDa observed; 591.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.