ABE432

Anti-BRDT Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Bromodomain testis-specific protein, Bromodomain-containing female sterile homeotic-like protein, RING3-like protein
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: mouse
• Technique(s): immunohistochemistry: suitable
• Numéro d'accès NCBI: NP_473395
• Numéro d'accès UniProt: Q91Y44
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Brdt(114642)

Description

Description générale

Bromodomain testis-specific protein (BRDT), also known as Bromodomain-containing female sterile homeotic-like protein, or RING3-like protein, and encoded by the gene BRDT/ FSRG3 is a testis-specific chromatin protein that binds specifically histone H4 acetylated at ′Lys-5′ and ′Lys-8′ (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis. Recent studies have shown that BRDT plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time. Therefore, it is involved in the late pachytene spermatocytes development. In the post-meiotic phase of spermatogenesis, BRDT binds to hyperacetylated histones and participates in their general removal from DNA. In addition, BRDT acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids, and participates in 3′-UTR truncation of specific mRNAs in post-meiotic spermatids. BRDT is required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin. BRDT is testis-specific, and expressed in the germinal cells: in the early meiotic (pachytene) spermatocytes during spermiogenesis, in the round and elongating spermatids , and in the condensed late spermatids.

Immunogène

GST-tagged recombinant protein corresponding to mouse BRDT.

Application

This Anti-BRDT antibody is validated for use in Immunohistochemistry for the detection of BRDT.

Qualité

Evaluated by Immunohistochemistry in mouse testis tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected BRDT in mouse testis tissue.

Description de la cible

107 kDa calculated

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable