AB9078

Anti-Ryanodine Receptor 1 Antibody

serum, Chemicon^®

• Synonyme(s) : Anti-CCO, Anti-KDS, Anti-MHS, Anti-MHS1, Anti-PPP1R137, Anti-RYDR, Anti-RYR, Anti-RYR-1, Anti-SKRR
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: serum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human, mouse, rat
• Fabricant/nom de marque: Chemicon^®
• Technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NM_000540.2; NM_001042723.1
• Numéro d'accès UniProt: P21817
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... RYR1(6261)

Description

Spécificité

Ryanodine Receptor 1.

Immunogène

Synthetic peptide from the variant TM region of human Ryanodine Receptor 1.

Application

Anti-Ryanodine Receptor 1 Antibody detects level of Ryanodine Receptor 1 & has been published & validated for use in IC, IH & WB.

Western blot: 1:1,000 using ECL. The antibody reacts with the 500 kDa Ryanodine Receptor 1 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel.

Immunocytochemistry: 1:1,000

Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.

Optimal working dilutions must be determined by the end user.

Remarque sur l'analyse

Control
Western blot = adult mouse skeletal muscle (adult mouse cardiac muscle will be negative).

IHC = adult mouse cerebellum Purkinje cells (adult mouse cerebellum granular cells will be negative).

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable