AB5613P
Anti-Brain Derived Neurotrophic Factor Antibody, pro
Chemicon®, from rabbit
Synonyme(s) :
Prepro-Abrineurin, Pro-Abrineurin, Prepro-BDNF, Pro-BDNF, Prepro-Brain-derived neurotrophic factor, Pro-Brain-derived neurotrophic factor
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
mouse, rat, human
Fabricant/nom de marque
Chemicon®
Technique(s)
immunohistochemistry: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... BDNF(627)
Description générale
Brain-derived neurotrophic factor (UniProt P23560; also known as Abrineurin, BDNF, Neurotrophin) is encoded by the BDNF (also known as BULN2) gene (Gene ID 627) in human. BDNF is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT3, and NT4/5. BDNF mediates its neurotrophic properties by signaling through the high affinity cell surface receptor gp145/trkB. BDNF is initially produced with a signal peptide (a.a. 1-18) and a propeptide (a.a. 19-128) sequence, the removal of which yields the mature BDNF (a.a. 129-247).
Spécificité
This polyclonal antibody detects prepro- and pro-BDNF, as well as prepro-/pro-BDNF-derived N-terminal fragments that contain the propeptide sequence (a.a. 19-128), but not mature BDNF or C-terminal fragments without the propeptide sequence. No cross-reactivity to other proneurotrophins.
Immunogène
Epitope: Propeptide.
KLH-conjugated linear peptide corresponding to a region within human BDNF propeptide sequence.
Application
Anti-Brain Derived Neurotrophic Factor Antibody, pro is an antibody proBDNF for use in Immunohistochemistry and Western Blotting.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
Neurochemistry & Neurotrophins
Neuroinflammation & Pain
Western blot: 1:2,000
Immunohistochemistry: 1:2,000
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:2,000
Optimal working dilutions must be determined by the end user.
Description de la cible
~35 kDa (glycosylated prepro-/pro-BDNF), ~50-65 kDa (glycosylated prepro-/pro-BDNF dimer), and ~26-28 kDa (non-glycosylated prepro/pro-BDNF or glycosylated prepro-/pro-BDNF N-terminal fragments) observed. Target bands appear larger than the calculated molecular weights of 27.82/28.87/29.82/37.12/31.12 kDa (human prepro-BDNF isoform 1/2/3/4/5) and 25.77/26.82/27.77/35.07/29.06 kDa (human pro-BDNF isoform 1/2/3/4/5) due to glycosylation and/or dimerization. Uncharacterized band(s) may appear in some lysates.
Forme physique
Lyophilized from 10 mM PBS. Reconstitute with 20 μL of sterile distilled water.
Stockage et stabilité
Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C undiluted for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (ACS grade or better) can be added (1:1) for additional stability.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
13 - Non Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Brain-derived neurotrophic factor rescues and prevents chronic intermittent hypoxia-induced impairment of hippocampal long-term synaptic plasticity.
Hui Xie,Kin-Ling Leung,Lei Chen,Ying-Shing Chan,Pak-Cheung Ng,Tai-Fai Fok,Yun-Kwok Wing et al.
Neurobiology of Disease null
Locomotor exercise alters expression of pro-brain-derived neurotrophic factor, brain-derived neurotrophic factor and its receptor TrkB in the spinal cord of adult rats.
Matylda Macias,Anna Dwornik,Ewelina Ziemlinska,Susanna Fehr,Melitta Schachner et al.
The European Journal of Neuroscience null
Kevin K Caldwell et al.
Pharmacology, biochemistry, and behavior, 90(4), 614-624 (2008-06-19)
Prenatal ethanol exposure is associated with an increased incidence of depressive disorders in patient populations. However, the mechanisms that link prenatal ethanol exposure and depression are unknown. Several recent studies have implicated reduced brain-derived neurotrophic factor (BDNF) levels in the
E W Neeley et al.
Neuroscience, 187, 24-35 (2011-04-19)
Psychiatric illness and anxiety disorders have strong neurodevelopmental components. Environmental insults such as prenatal exposure to stress and genetic differences in stress responses may affect brain development. A rat model of random variable prenatal stress was used to study the
Oscar V Torres et al.
The international journal of neuropsychopharmacology, 21(3), 281-290 (2017-11-23)
The continuing epidemic of methamphetamine addiction has prompted research aimed at understanding striatal dysfunctions potentially associated with long-term methamphetamine use. Here, we investigated transcriptional and translational alterations in the expression of neurotrophic factors in the rat striatum at 30 days
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