AB5154
Anti-Cav2.2 Antibody
Chemicon®, from rabbit
Synonyme(s) :
Voltage Gated Calcium Channel (N-type) alpha 1B Subunit, Cacna1b
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
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Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
rat
Fabricant/nom de marque
Chemicon®
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (tissue)
immunoprecipitation (IP): suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
rat ... Cacna1B(257648)
Spécificité
Recognizes a 1B subunit (both LMW and HMW forms) of voltage-gated calcium channel. Does not cross react with any other calcium channel antigens tested so far.
SPECIES REACTIVITIES: It is expected that the antibody will also work on mouse due to sequence homology (15/17). Other species have not been tested.
SPECIES REACTIVITIES: It is expected that the antibody will also work on mouse due to sequence homology (15/17). Other species have not been tested.
Immunogène
Purified peptide CNB1 from 851-867 of alpha 1B subunit of rat brain voltage-gated calcium channel (Accession Q02294; Westenbroek et al. 1992) containing N-terminal lysine and tyrosine, conjugated to KLH with gluteraldehyde (Harlow & Lane 1988).
Application
Detect Cav2.2 using this Anti-Cav2.2 Antibody validated for use in IC, IP & WB.
In preparing full-length protein samples for Western blot or immunoprecipitation, the procedures should be performed at 4°C, with the following protease inhibitor mixture: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).
Immunoprecipitation
Immunocytochemistry. Recommend subsequent staining with a labeled second antibody.
Western blot: 1.5 to 3.0 μg/mL (1:100-1:200) using ECL
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Immunoprecipitation
Immunocytochemistry. Recommend subsequent staining with a labeled second antibody.
Western blot: 1.5 to 3.0 μg/mL (1:100-1:200) using ECL
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Neuroinflammation & Pain
Ion Channels & Transporters
Neuroinflammation & Pain
Forme physique
Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Stockage et stabilité
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at 2-8°C in undiluted aliquots for up to 2 weeks. For longer periods, it is recommended to add glycerol (ACS grade or better) at a ratio of 1:1 and store the antibody at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Remarque sur l'analyse
Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 10 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 10 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
J H Xu et al.
Neuropathology and applied neurobiology, 36(1), 71-85 (2009-10-09)
To investigate the subcellular localization of Ca(v)2.2 calcium channel in the mouse central nervous system (CNS), and changes of Ca(v)2.2 at acute and chronic stages during and after pilocarpine-induced status epilepticus (PISE), in order to find out the roles it
Kang Zhang et al.
Frontiers in pharmacology, 8, 302-302 (2017-06-13)
The sigma-1 receptor is a 223 amino acids molecular chaperone with a single transmembrane domain. It is resident to eukaryotic mitochondrial-associated endoplasmic reticulum and plasma membranes. By chaperone-mediated interactions with ion channels, G-protein coupled receptors and cell-signaling molecules, the sigma-1
R E Westenbroek et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 12(6), 2259-2267 (1992-06-01)
Type I and type III Na+ channels are localized mainly in neuronal cell bodies in mouse brain. Type II channels are preferentially localized in unmyelinated fiber tracts but are not detectable in normally myelinated fibers. In shiverer mice, which lack
Hang Zhou et al.
Molecular brain, 8, 42-42 (2015-07-18)
The density of functional synapses is an important parameter in determining the efficacy of synaptic transmission. However, how functional presynaptic terminal density is regulated under natural physiological conditions is still poorly understood. We studied the factors controlling the density of presynaptic
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