AB5074P

Anti-Amyloid Antibody, β 1-40

Chemicon^®, from rabbit

• Synonyme(s) : Anti-AAA, Anti-ABPP, Anti-AD1, Anti-APPI, Anti-CTFgamma, Anti-CVAP, Anti-PN-II, Anti-PN2, Anti-alpha-sAPP, Anti-preA4
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: mouse, human
• Fabricant/nom de marque: Chemicon^®
• Technique(s): ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NM_000484.2; NM_201413.1; NM_201414.1
• Numéro d'accès UniProt: P05067
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... APP(351)

Description

Description générale

The cerebral and vascular plaques associated with Alzheimer′s disease (AD) are mainly composed of amyloid beta peptides (Ab). Ab is derived from cleavage of the amyloid precursor protein (APP) and varies in length from 39 to 43 amino acids. Ab [1-40], Ab [1-42], and Ab [1-43] peptides result from cleavage of APP after residues 40, 42, and 43, respectively. The cleavage takes place by gamma-secretase during the last APP processing step. Ab [1-40], [1-42] and [1-43] peptides are major constituents of the plaques and tangles that occur in AD. Ab antibodies and peptides have been developed as tools for elucidating the biology of AD.

Spécificité

Recognizes beta-amyloid 1-40. One of the most important and initial steps which causes loss of memory and cognition in Alzheimer′s Disease (AD) involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43 amino acid peptides (beta amyloid 1-40, 1-42 and 1-43). Polymerization of beta-amyloid and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition. The immunogen peptide shows homology with beta-amyloid 1-28 and beta-amyloid 12-28. No cross reactivity is observed with CGRP.

Immunogène

A 7 amino acid peptide sequence from the C terminus of human beta-amyloid 1-40.

Application

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

This Anti-Amyloid Antibody, β 1-40 is validated for use in ELISA, IH, WB for the detection of Amyloid.

Western blot: 1-10 μg/mL (Chemiluminescence technique)

Immunohistochemistry: 10-50 μg/mL using formalin or paraformaldehyde fixed Alzheimer′s brain tissue.

ELISA: 1:10,000-1:100,000 (50-100 ng immunogen peptide/well)

Optimal working dilutions must be determined by the end user.

Forme physique

Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA, no preservative.

ImmunoAffinity Purified

Stockage et stabilité

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable