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AB1284

Sigma-Aldrich

Anti-Heme Oxygenase 1 Antibody

Chemicon®, from rabbit

Synonyme(s) :

HO-1, HSP32

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

affinity purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

bovine, mouse, rat

Ne doit pas réagir avec

canine

Fabricant/nom de marque

Chemicon®

Technique(s)

immunohistochemistry: suitable
western blot: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

bovine ... Hmox1(513221)
dog ... Hmox1(442987)
mouse ... Hmox1(15368)
rat ... Hmox1(24451)

Spécificité

Recognizes Heme oxygenase-1.

Immunogène

A synthetic peptide with the amino acid sequence H-D-L-S-E-A-L-K-E-A-T-K-E-V-H-C-NH2 corresponding to amino acid residues [12-25] + Cys-NH2 of the human HO-1 protein (Yoshida et al., 1988).

Application

Research Category
Metabolism
Research Sub Category
Toxicology & Drug Resistance
This Anti-Heme Oxygenase 1 Antibody is validated for use in IH, WB for the detection of Heme Oxygenase 1.
Western Blot: 1:1,000 (using ECL). A single band of ~32 kD is observed. Reactivity can be completely abolished by pre-adsorption of the antibody with the complementary peptide (Catalog Number AG253). Also available are microsomal tissue preparations for control purposes (Rat spleen microsomal preparation, Catalog Number AG257).

Immunohistochemistry: 1:50-1:1,000 on human airway biopsy, human spleen, human kidney and porcine arteries.

Optimal working dilutions must be determined by the end user.

Description de la cible

32 kDa

Forme physique

Affinity Purified immunoglobulin. Liquid in PBS containing 0.01M sodium azide.
ImmunoAffinity Purified

Stockage et stabilité

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Remarque sur l'analyse

Control
Lung

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Resveratrol increases vascular oxidative stress resistance.
Ungvari, Z; Orosz, Z; Rivera, A; Labinskyy, N; Xiangmin, Z; Olson, S; Podlutsky, A; Csiszar, A
American Journal of Physiology. Heart and Circulatory Physiology null
Heme oxygenase-1 could mediate the protective effects of hyperbaric oxygen preconditioning against hepatic ischemia-reperfusion injury in rats.
Yi Liu,Xue-Jun Sun,Ji Liu,Zhi-Min Kang,Xiao-Ming Deng
Clinical and Experimental Pharmacology & Physiology null
Purification and properties of heme oxygenase from pig spleen microsomes
Yoshida T, and Kikuchi G.
The Journal of Biological Chemistry, 253, 4224-4229 (1978)
Solubilization and partial purification of heme oxygenase from rat liver.
Maines, M D, et al.
The Journal of Biological Chemistry, 252, 5900-5903 (1977)
Up-regulation of heme oxygenase-1 by isoflurane preconditioning during tolerance against neuronal injury induced by oxygen glucose deprivation.
Qifang Li, Yesen Zhu, Hong Jiang, Hui Xu, Heping Liu
Acta biochimica et biophysica Sinica null

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