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8.55054

Sigma-Aldrich

EDANS NovaTag®_resin

Novabiochem®

Synonyme(s) :

EDANS-MPB-AM resin

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About This Item

Code UNSPSC :
12352116
Nomenclature NACRES :
NA.22

Niveau de qualité

200

Gamme de produits

NovaSyn® TG
Novabiochem®

Forme

beads

Capacité de réaction

reaction type: Fmoc solid-phase peptide synthesis

Fabricant/nom de marque

Novabiochem®

Application(s)

peptide synthesis

Groupe fonctionnel

amine

Température de stockage

15-25°C

Description générale

The EDANS/dabcyl fluorophore-quencher pair is one of the most commonly-used for FRET peptides, owing to excellent spectral overlap between the emission spectrum of EDANS and absorbance spectrum of dabcyl [1]. The introduction of the EDANS moiety is traditionally achieved either by coupling of a peptide fragment to EDANS in solution [1, 2] or through the use of a pre-formed derivative, such as Fmoc-Glu(EDANS)-OH [3]. Novabiochem′s EDANS resin enables for the first time the direct synthesis of C-terminally EDANS-labeled peptides by solid phase synthesis [4]. Loading of the first residue requires acylation of the resin-bound secondary amine and is, therefore, best carried out using HATU activation. Chain extension and cleavage can then be effected using standard methods. EDANS NovaTag resin has been recently employed to prepare fluorescently labeled aminoalkane diphenyl phosphonate affinity probes for chymotrypsin- and elastase-like serine proteases [5].

Associated Protocols and Technical Articles
Protocols for Loading of Peptide Synthesis Resins Literature references

[1] G. T. Wang, et al. (1990) Tetrahedron Lett., 31, 6493.
[2] C. Garcia-Echeverria & D. H. Rich (1992) Febs Lett., 297, 100.
[3] J. W. Drijfhout, et al. in ′Peptides, Chemistry, Structure& Biology, Proc. 14th American Peptide Symposium′, P. T. P. Kaumaya &R. S. Hodges (Eds), Kingswinford, Mayflower Scientific Ltd., 1996, pp. 129.
[4] J. Beythien & P. White (2005) Tetrahedron Lett., 46,101.
[5] B. F. Gilmore, et al. (2009) Bioconjugate Chem., 20, 2098.

Liaison

Replaces: 04-12-3904

Remarque sur l'analyse

Color (visual): white to yellow to brown
Appearance of substance (visual): beads
Loading (determined from the substitution of the Fmoc-Leu loaded resin): 0.30 - 0.60 mmol/g
Swelling Volume (in DMF): lot specific result
The polymer matrix is copoly styrene - 1 & DVB 100 - 200 mesh

Informations légales

NOVASYN is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVATAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Articles

Peptide-Labeling

The spectral properties of Novabiochem®’s chromogenic and fluorogenic derivatives are invaluable tools for biochemistry, having numerous applications in enzymology, protein chemistry, immunology and histochemistry. Novabiochem® range offers one of the most extensive ranges of labeling reagents for the synthesis of such peptides, including the unique NovaTag™ resins for the production of C-terminally-labeled peptide

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