71340-M

pCDFDuet-1 DNA - Novagen

• Synonyme(s) : Duet-1 DNA, Novagen DNA Vector, pCDFDuet-1 DNA
• Code UNSPSC : 41106602
• Nomenclature NACRES : NA.51

Propriétés

• Forme: liquid
• Niveau de qualité: 100
• Fabricant/nom de marque: Novagen^®
• Conditions de stockage: OK to freeze
• Promoteur: Promoter name: T7
• selection: spectinomycin
• Température de stockage: −70°C

Description

Description générale

pCDFDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker. This vector can be transformed into the same cell with pETDuet-1, pACYCDuet-1, and pRSFDuet-1 or pCOLADuet-1 for the coexpression of up to eight target genes. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.

Application

pCDFDuet-1 DNA has been used as a vector for the expression of recombinant proteins in E-coli cells.

Avertissement

Toxicity: Standard Handling (A)

Informations légales

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable