70527

100 mM IPTG Solution - Novagen

Convenient, ready-to-use solution

• Synonyme(s) : 100 mM IPTG Solution - Novagen, IPTG Solution, 100 mM, Isopropyl -β-D-1-thiogalactopyranoside, Isopropylthio -β-D-galactoside
• Numéro CAS: 367-93-1
• Numéro MDL: MFCD00063273
• Code UNSPSC : 12352200

Propriétés

• Niveau de qualité: 100
• Forme: solution
• Poids mol.: 238.30 g/mol
• Fabricant/nom de marque: Novagen^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• InChI: 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
• Clé InChI: BPHPUYQFMNQIOC-NXRLNHOXSA-N

Description

Application

100 mM IPTG Solution - Novagen has been used to:

• induce expression of an anti-CD11b-nanobody with a sortase recognition tag in Chalmers (WK6) cells
• induce expression in calcium-independent sortase from BL21(DE3) cells
• turn on bacterial protein production of the protein A/G-MNase

Actions biochimiques/physiologiques

Isopropyl-β-D-1-thiogalactopyranoside (IPTG) is an inducer of the lacoperon. It is highly recommended for blue/white screening using lacZα-complementation in appropriate vectors and host strains, for example, pSTBlue-1/NovaBlue. In addition, it is also used for inducing protein expression with the pET system and various lac promoter-derived expression systems. IPTG is supplied as a sterile aqueous solution and is free of dioxane. It comes in convenient volumes of 10 × 1.5 ml. IPTG is used in bacterial cultures to induce the expression of plasmid-based genes to produce recombinant proteins under the control of the lac promoter.

Avertissement

Toxicity: Carcinogenic / Teratogenic (D)

Informations légales

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable