444248

MMP-13, Proenzyme, His•Tag^®, Human, Recombinant, S. frugiperda

• Synonyme(s) : Collagenase-3
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Pureté: ≥90% (SDS-PAGE)
• Niveau de qualité: 100
• Forme: liquid
• Activité spécifique: ≥50 mU/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Conditions d'expédition: wet ice
• Température de stockage: −70°C

Description

Description générale

Full-length recombinant, human MMP-13 fused to a His•Tag sequence and expressed in S. frugiperda insect cells. This 452 amino acid proenzyme contains an N-terminal propeptide which confers latency to the proenzyme, a Ca²⁺- and Zn²⁺- binding catalytic domain, a hinge region, and a C-terminal hemopexin domain. Hydrolyzes collagen type II 5-6 times faster than collagens type I and III. Exhibits high activity towards gelatin and degrades α1-antichymotrypsin and plasminogen activator inhibitor-2.

Full-length recombinant, human MMP-13 fused to a His•Tag sequence and expressed in S. frugiperda insect cells. This 452 amino acid proenzyme contains the N-terminal propeptide, the Ca²⁺- and Zn²⁺-binding catalytic domain, the hinge region, and the C-terminal hemopexin domain. Hydrolyzes collagen type II 5 to 6 times faster than collagens Type I and III. Exhibits high activity towards gelatin and degrades α₁-antichymotrypsin and plasminogen activator inhibitor-2 (PAI-2).

Note: 1 mU = 1 milliunit.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of APMA-activated enzyme that will hydrolyze 1.0 µmol MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂ (Cat. No. 03-32-5032) per min at 37°C, pH 7.5.

Forme physique

In 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Autres remarques

Herget, J., et al. 2003. Am. J. Physiol.285, 4199.
Nagase, H. 1997. Biol. Chem.378, 151.
Knäuper, V., et al. 1996. J. Biol. Chem. 271, 1544.
Knauper, V., et al. 1996. J. Biol. Chem.271, 17124.
Pei, D., and Weiss, S.J. 1996. J. Biol. Chem.271, 9135.
Knight, C.G., et al. 1992. FEBS Lett.296, 263.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable