251760

DAR-4M

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.

• Synonyme(s) : DAR-4M, Diaminorhodamine-4M
• Formule empirique (notation de Hill): C₂₅H₂₆N₄O₃
• Poids moléculaire : 430.50
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.25

Propriétés

• Niveau de qualité: 100
• Pureté: ≥98% (HPLC)
• Forme: solution
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Couleur: red-violet
• Fluorescence: λ_ex ~560 nm; λ_em ~575 nm
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of O₂, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH, unlike DAF-2 (Cat. No. 251500).

A rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. Unlike DAF-2 (Cat. No. 251500), its fluorescence intensity is not dependent on the pH. Excitation maximum: ~560 nm; emission maximum: ~575 nm.

Actions biochimiques/physiologiques

Cell permeable: no

Primary Target
Nitric oxide (NO) fluorescent indicator

Product does not compete with ATP.

Reversible: no

Avertissement

Toxicity: Irritant (B)

Forme physique

In DMSO.

Reconstitution

Following initial thaw, aliquot and freeze at (-20°C). Use immediately following dilution and discard any unused portion.

Autres remarques

Kojima, H., et al. 2001. Anal. Chem.73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett.41, 69.

Informations légales

Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): 188.6 °F
• Point d'éclair (°C): 87 °C