23-030-M

SCF-Fbw7 Protein Complex, Active, 10 µg

Active complex of four (4) full length, recombinant human enzymes: Fbw7, Skp1, Cul1 & Rbx1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.

• Synonyme(s) : SCF ^Fbxw7, SCF ^hCdc4
• Code UNSPSC : 12352202
• eCl@ss : 32160405
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: human
• Niveau de qualité: 100
• Produit recombinant: expressed in baculovirus infected Sf21 cells
• Fabricant/nom de marque: Upstate^®
• Technique(s): activity assay: suitable
• Numéro d'accès NCBI: NM_003592; NM_014248; NM_033632; NM_170679
• Informations sur le gène: human ... Cul1(8454) , Fbw7(55294) , Rbx1(9978) , Skp1(6500)

Description

Description générale

N-terminal c-Myc, 6His-tagged, recombinant human Smad7 full length, expressed by baculovirus in Sf21 insect cells.

The SCF (Skp1-Cul1-F-box protein) complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. The SCF consists of three invariant components, Skp1, Cul1, and Rbx1 and an interchangeable subunit, an F-box protein which is responsible for recruiting specific substrates to be ubiquitinated by the SCF. SCF^Fbw7 mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell division, growth and differentiation, including Cyclin E1, c-Myc, Notch and c-Jun. Mutations have been found in the Fbw7 subunit of SCF^Fbw7 in various types of human cancers, therefore Fbw7 is considered to be a potent tumour suppressor.

Application

Ubiquitination Cascade Comoponent: E3

Forme physique

Purified using glutathione sepharose.

Autres remarques

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.