17-10495
EZ-Magna ChIRP® RNA Interactome Kit
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About This Item
Code UNSPSC :
41105331
Nomenclature NACRES :
NA.84
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Niveau de qualité
Description générale
The Magna ChIRP RNA Interactome Kit uses the ChIRP method (Chromatin Isolation by RNA Purification) to enable identification and analysis of regions of chromatin that interact with a particular chromatin-associated RNA. This novel anti-sense probe-based capture strategy uses cross-linked chromatin to allow unbiased discovery of RNA-associated DNA sequences and proteins. To perform ChIRP two pools consisting of mulitple biotinylated oligonucleotide probes complementary to the RNA of interest are used (see figure 1). These probe sets are combined with chromatin and hybridized to the chromatin-associated RNA. Complexes containing biotinylated-probes bound to the chromatin-associated RNA are then isolated using streptavidin magnetic beads. DNA or RNA can then be recovered and analyzed by quantitative PCR or next generation sequencing (ChIRP-seq). Alternatively, proteomic analysis of the recovered chromatin can be performed.
Application
The EZ-Magna ChIRP RNA Interactome Kit allows analysis, and mapping of regions of chromatin interacting with chromatin-associated RNAs. Based on the ChIRP method (chromatin isolation by RNA purification), this probe-based capture assay enables discovery of RNA-associated DNA sequences and proteins.
Conditionnement
Kit capacity: 12 Chromatin Isolation by RNA Purification Assays (includes negative control probes, even and odd positive control TERC lncRNA probes, and control PCR primer sets)
Composants
10X Glycine ;Lysis Buffer;Hybridization Buffer (without 15% formamide) ;Wash Buffer;Proteinase K Buffer (for DNA);Proteinase K Buffer (for RNA);DNA Elution Buffer;0.5M EDTA;Streptavidin Magnetic Beads;RNase Inhibitor;Protease Inhibitor Cocktail III;Proteinase K Solution;DNase I RNase free;DNase I Reaction Buffer;RNase A (20 mg/mL);RNase H (10U/ μL);Magna ChIRP Negative Control Probe Set (LacZ) ;Magna ChIRP TERC lncRNA Probe Set (Even) ;Magna ChIRP TERC lncRNA Probe Set (Odd) ;RNA Positive Control Primers (TERC Gene);RNA Negative Control Primers (GAPDH Gene);Magna ChIRP Primers, WNT-1 precursor ;ChIP Primers, GAPDH coding D2
Informations légales
MAGNA CHIRP is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Resp. Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
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