116816

Adenosine 3′,5′-cyclic Monophosphorothioate, 8-Bromo-, Rp-Isomer, Sodium Salt

A potent, cell-permeable, and reversible metabolically-stable cAMP antagonist that inhibits cAMP-dependent protein kinase and shows preference for PKA type I.

• Synonyme(s) : Adenosine 3′,5′-cyclic Monophosphorothioate, 8-Bromo-, Rp-Isomer, Sodium Salt, Rp-8-Br-cAMPS, Na, PKA Inhibitor VI
• Formule empirique (notation de Hill): C₁₀H₁₀BrN₅O₅PS · Na
• Poids moléculaire : 446.15
• Code UNSPSC : 41106305
• Nomenclature NACRES : NA.77

Propriétés

• Niveau de qualité: 100
• Pureté: ≥99% (HPLC)
• Forme: lyophilized
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated (hygroscopic)
• Solubilité: water: 40 mg/mL
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

A potent, cell-permeable, and reversible metabolically-stable cAMP antagonist that inhibits cAMP-dependent protein kinase and shows preference for PKA type I. More lipophilic than cAMP antagonist Rp-cAMPS (Cat. No. 116814).

Cell-permeable, potent, reversible, and metabolically stable antagonist of cAMP that is resistant to the action of mammalian cyclic nucleotide phosphodiesterases. Rp-8-Br-cAMPS is more lipophilic as compared to 8-Br-cAMP and Rp-cAMPS. In contrast to other common ATP-site inhibitors or to its parent compound Rp-cAMPS, Rp-8-Br-cAMPS discriminates between both isozymes of protein kinase A, preferring type I, thus providing additional selectivity. By occupying cAMP binding sites, prevents dissociation and activation of the kinase holoenzyme. λ = 264 nm (H₂O, pH 7.0); ε_max 17,000 M^-1cm^-1.

Note: 5 µmol = 2.23 mg.

Actions biochimiques/physiologiques

Cell permeable: yes

Primary Target
PKA 1

Product does not compete with ATP.

Reversible: yes

Avertissement

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Autres remarques

Calaghan, S.C., et al. 1999. Pflugers Arch. 437, 780.
Gjertsen, B.T., et al. 1995. J. Biol. Chem. 270, 20599.
Yokozaki, H., et al. 1992. Cancer Res.52, 2504.
Dostmann, W.R. et al. 1990. J. Biol. Chem.265, 10484.

Informations légales

Sold under license of Patent DE 3,802,865.4 issued to BIOLOG LSI.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable