1.15940

Crystal violet (C.I. 42555)

for microscopy Certistain^®

• Synonyme(s) : Crystal violet (C.I. 42555), Hexamethylenepararosaniline chloride, Gentian violet (C.I. 42555), Hexamethyl-p-rosanilinium, Methyl violet 10 B
• Formule empirique (notation de Hill): C₂₅H₃₀ClN₃
• Numéro CAS: 548-62-9
• Poids moléculaire : 407.98
• Numéro MDL: MFCD00011750
• Code UNSPSC : 41116130
• Numéro de classement CE : 208-953-6

Propriétés

• Niveau de qualité: 400
• Forme: solid
• Puissance: 420 mg/kg LD₅₀, oral (Rat)
• Composition: Dye, ≥88% spectrophotometric assay
• pH: 2.5-3.5 (20 °C, 10 g/L in H₂O)
• Pf: 189-194 °C
• Solubilité: 10 g/L
• Densité: 1.19 g/cm³ at 20 °C
• Masse volumique apparente: 220‑400 kg/m³
• εmax: 2050-2450 at 589-594 nm in water at 0.002 g/L
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: 2-30°C
• InChI: 1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
• Clé InChI: ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Description

Description générale

Crystal violet (C.I. 42555) for microscopy Certistain^®, is a dry dye that can be used to produce a Crystal violet staining solution to differentiate Gram-positive and Gram-negative bacteria in samples of human origin. In the case of Gram-positive bacteria, the bacteria retain their dark blue / dark purple color even after the treatment with decolorizing solution. On the other side Gram-negative bacteria are stained red or pink with the counterstain. The Certistain^® dry dye can be used for clinical diagnostic purposes as it is certified and registered as IVD and CE product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Remarque sur l'analyse

Dye content (spectrophotometrically): ≥ 88%
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (water): 589 - 594 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.002 g/l; water): 2050 - 2450
TLC-Test: passes test
Michler`s ketone: <0.10%
Loss on drying (110 °C): ≤ 10%
Suitability for microscopy: passes test

Informations légales

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS05,GHS07,GHS08,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H302 - H318 - H351 - H410
• Conseils de prudence: P201 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Classification des risques: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3