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Key Documents

09-071

Sigma-Aldrich

Anti-G9a (BAT8) Antibody

from rabbit, purified by affinity chromatography

Synonyme(s) :

G9A histone methyltransferase, H3-K9-HMTase 3, HLA-B associated transcript 8, HLA-B-associated transcript 8, Histone H3-K9 methyltransferase 3, Lysine N-methyltransferase 1C, Protein G9a, ankyrin repeat-containing protein, chromosome 6 open reading frame

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

human, mouse

Réactivité de l'espèce (prédite par homologie)

canine, rat

Technique(s)

western blot: suitable

Numéro d'accès NCBI

NP_006700.3

Numéro d'accès UniProt

Q96KQ7

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... EHMT2(10919)
mouse ... Ehmt2(110147)
rat ... Ehmt2(361798)

Description générale

G9a (BAT8 or Histone-lysine N-methyltransferase) is thought to be involved in intracellular protein-protein interaction and is a lysine-preferring histone methyltransferase. H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. The presence of the ankyrin repeats also suggests that G9a is involved in intracellular protein-protein interaction. Like Suv39h1, G9a transfers methyl groups to the lysine residues of histone H3, but with a 10- to 20-fold higher activity. G9a is localized to the nucleus but not in the repressive chromatin domains of centromeric loci, in which Suv39h1 family proteins were localized. This protein is probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. G9a also methylates histone H1.

Spécificité

This antibody recognizes G9a (BAT8).

Immunogène

KLH-conjugated linear peptide corresponding to G9a (BAT8).

Application

Anti-G9a (BAT8) Antibody is a rabbit polyclonal antibody for detection of G9a (BAT8) also known as G9A histone methyltransferase ,H3-K9-HMTase 3 ,HLA-B associated transcript 8 & has been validated in WB.
Western Blot Analysis (SNAP ID Technique): 1 µg/mL from a previous lot detected G9a (BAT8) on 10 µg of HeLa cell lysate.

Qualité

Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: : 0.25-2 µg/mL of this antibody detected G9a (BAT8) in 10 µg of HeLa cell lysate.

Description de la cible

~160 kDa

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Shivakumar Subbanna et al.
Neurobiology of disease, 54, 475-485 (2013-02-12)
Rodent exposure to binge-like ethanol during postnatal day 7 (P7), which is comparable to the third trimester of human pregnancy, induces neuronal cell loss. However, the molecular mechanisms underlying these neuronal losses are still poorly understood. Here, we tested the
S Subbanna et al.
Neuroscience, 258, 422-432 (2013-12-05)
The transient exposure of immature rodents to ethanol during postnatal day 7 (P7), comparable to a time point within the third trimester of human pregnancy, induces neurodegeneration. However, the molecular mechanisms underlying the deleterious effects of ethanol on the developing
Sang Eun Park et al.
Oncotarget, 7(26), 39796-39808 (2016-10-26)
We previously reported that BIX-01294 (BIX), a small molecular inhibitor of euchromatic histone-lysine N-methyltransferase 2 (EHMT2/G9a), induces reactive oxygen species (ROS)-dependent autophagy in MCF-7 cells. Herein, we analyzed the epigenetic mechanism that regulates the transcription of Beclin-1, a tumor suppressor
Lifeng Wang et al.
The EMBO journal, 32(1), 45-59 (2012-11-28)
PPARγ promotes adipogenesis while Wnt proteins inhibit adipogenesis. However, the mechanisms that control expression of these positive and negative master regulators of adipogenesis remain incompletely understood. By genome-wide histone methylation profiling in preadipocytes, we find that among gene loci encoding
Ai-Shun Guo et al.
Molecular medicine reports, 14(5), 4613-4621 (2016-10-26)
The present study aimed to investigate the differential expression and clinical significance of histone methyltransferase G9a, histone H3K9me2 and histone H3K9me1 in human brain glioma and adjacent tissue samples. It also aimed to observe the effect and mechanism of BIX‑01294, as
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