07-520

Anti-eNOS/NOS III Antibody, CT

Upstate^®, from rabbit

• Synonyme(s) : Constitutive NOS, Endothelial NOS, Endothelial NOS, Endothelial NOS, endothelial nitric oxide synthase, nitric oxide synthase 3 (endothelial cell)
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: mouse, pig, guinea pig, rat, canine, human, bovine
• Fabricant/nom de marque: Upstate^®
• Technique(s): western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NP_000594
• Numéro d'accès UniProt: P29474
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: bovine ... Nos3(287024) ; dog ... Nos3(403784) ; guinea pig ... Nos3(100135577) ; human ... NOS3(4846) ; mouse ... Nos3(18127) ; pig ... Nos3(397557) ; rat ... Nos3(24600)

Description

Description générale

Endothelial NOS (eNOS), also known as Nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is a nitric oxide synthase. It generates NO in blood vessels and is involved with regulating vascular function. A constitutive Ca2+ dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of Golgi bodies within cells.

Spécificité

Predicted to cross-react with mouse, guinea pig, pig, rat and dog based on sequence homology.

Recognizes eNOS/NOS III, Mr ~135 kDa.

Immunogène

KLH-conjugated, synthetic peptide corresponding to amino acids 1181-1194 (C-QERQLRGAVPWAFD) of human eNOS/NOS III. The immunizing sequence has 10/11 identical amino acids in mouse, guinea pig, bovine, pig, rat, and dog.

Application

Anti-eNOS/NOS III Antibody, CT detects level of eNOS/NOS III & has been published & validated for use in WB.

Qualité

Routinely evaluated by western blot in RIPA lysates from HUVEC cells.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected eNOS/NOS III in RIPA lysates from HUVEC cells.

Description de la cible

135 kDa

Forme physique

Format: Purified

Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.

Remarque sur l'analyse

Control
Platelets, placenta, liver and kidney.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1