05-802R
Anti-phospho-Akt (Thr308) Antibody, clone 50-1C-25, rabbit monoclonal
clone NL50, Upstate®, from rabbit
Synonyme(s) :
Protein kinase B, RAC-alpha serine/threonine-protein kinase, murine thymoma viral (v-akt) oncogene homolog-1, rac protein kinase alpha, v-akt murine thymoma viral oncogene homolog 1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
NL50, monoclonal
Espèces réactives
human, bovine, mouse, rabbit, chicken
Fabricant/nom de marque
Upstate®
Technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
western blot: suitable
Isotype
IgG
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
phosphorylation (pThr308)
Informations sur le gène
bovine ... Akt1(280991)
human ... AKT1(207)
Description générale
Akt/PKB is a Ser/Thr kinase and a major known effecter of the PI3 Kinase pathway. It is involved in multiple signaling pathways that relate to many biological processes including metabolism, apoptosis, cell cycle control, angiogenesis, differentiation, and cell growth and proliferation. In mammals, three isoforms of Akt (Akt1/PKCα, Akt2/PKBβ, and Akt3/PKBγ) exists. They exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt1 is the predominant isoform that is in most tissues and is thought to have a dominant role in growth, survival, embryonic development, and adipocyte differentiation. Akt2 is correlated with the regulation of glucose homeostasis and is the predominant isoform expressed in insulin-responsive tissues. Akt3 is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal Pleckstrin Homology (PH) domain that provides a lipid-binding module, a central catalytic domain containing Thr308, and a C-terminal hydrophobic motif containing Ser473. The activation of AKT is dependent on a dual regulatory mechanism that requires both its translocation to the plasma membrane and dual phosphorylation on Thr308 and Ser473 by PDK1 and the TORC2 complex, respectively.
Spécificité
Detects Akt only when phosphorylated on Thr308.
Immunogène
KLH-conjugated synthetic peptide containing the Thr308 sequence and surrounding amino acids of human, mouse, rat, bovine and chicken Akt1.
Application
Detect phospho-Akt (Thr308) using this Anti-phospho-Akt (Thr308) Antibody, clone 50-1C-25 validated for use in WB, IF, IC, ELISA.
Research Category
Signaling
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
PI3K, Akt, & mTOR Signaling
Western Blot Analysis:
This lot detected Akt when phosphorylated on Thr308 at 1:1,000 dilution in PDGF-treated NIH/3T3 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).
This lot detected Akt when phosphorylated on Thr308 at 1:1,000 dilution in PDGF-treated NIH/3T3 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).
Qualité
This lot detected Akt when phosphorylated on Thr308 at 1:1,000 dilution in PDGF-treated NIH/3T3 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).
Description de la cible
60 kDa
Liaison
Replaces: 05-802
Forme physique
Format: Purified
Protein A purified
Protein A purified recombinant rabbit monoclonal supernatant in 0.1M Tris-Glycine (pH7.4) 150mM NaCl, and 0.05% NaN3 with 40% glycerol.
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Scott Ackler et al.
Oncogene, 21(2), 198-206 (2002-01-23)
AKT1/protein kinase Balpha is a protein-serine/threonine kinase that regulates multiple targets involved in cell survival and cell cycle progression in a variety of cell types including breast cancer cells. To explore the role of Akt1 in mammary gland function and
D R Alessi et al.
The EMBO journal, 15(23), 6541-6551 (1996-12-02)
Insulin activated endogenous protein kinase B alpha (also known as RAC/Akt kinase) activity 12-fold in L6 myotubes, while after transfection into 293 cells PKBalpha was activated 20- and 50-fold in response to insulin and IGF-1 respectively. In both cells, the
S R James et al.
The Biochemical journal, 315 ( Pt 3), 709-713 (1996-05-01)
Recent evidence has suggested that activation of phosphoinositide 3-kinase (PI 3-kinase) is required for the activation of Akt-1 by growth factors and insulin. Here we demonstrate by two independent methods that Akt-1 from L6 myotubes binds to PtdIns(3,4,5)P3, PtdIns(3,4)P2 and
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