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Merck
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Key Documents

05-644

Sigma-Aldrich

Anti-CAR Antibody, clone RmcB

clone RmcB, Upstate®, from mouse

Synonyme(s) :

46 kD coxsackievirus and adenovirus receptor (CAR) protein, CVB3 binding protein, CVB3-binding protein, Coxsackievirus B-adenovirus receptor, coxsackie virus B receptor, coxsackie virus and adenovirus receptor

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

RmcB, monoclonal

Espèces réactives

human, rat, mouse

Fabricant/nom de marque

Upstate®

Technique(s)

immunocytochemistry: suitable
western blot: suitable

Isotype

IgG1

Numéro d'accès NCBI

NM_001338

Numéro d'accès UniProt

P78310

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CXADR(1525)

Description générale

The coxsackievirus and adenovirus receptor (CAR) mediates cell attachment and infection by coxsackie B viruses and by a number of adenoviruses. CAR also mediates homotypic intercellular interactions. In polarized epithelial cells, CAR is closely associated with the tight junction, where it contributes to the barrier to paracellular flow of solutes and macromolecules. CAR′s biological roles are not well defined, but emerging evidence suggests that it may function during embryonic development and in regulating cell proliferation.

Spécificité

Recognizes CAR at MW ~46 kDa.

Immunogène

Native human coxsackie virus-adenovirus receptor protein.

Application

Anti-CAR Antibody, clone RmcB detects level of CAR & has been published & validated for use in IC & WB.
Immunocytochemistry:
1-2 μg/mL of a previous lot showed positive immunostaining for CAR in HeLa cells, hCAR transfected CHO cells, but not in untransfected CHO cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X.

Qualité

Evaluated by Western Blot on mouse small intestine lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected Car on 10 µg of mouse small intestine lysates.

Description de la cible

~46 kDa

Forme physique

Format: Purified
Purified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl and 0.05% Sodium Azide, pH 7.4.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Klaus Heger et al.
European journal of immunology, 45(6), 1614-1620 (2015-03-20)
Replication-deficient recombinant adenoviruses are potent vectors for the efficient transient expression of exogenous genes in resting immune cells. However, most leukocytes are refractory to efficient adenoviral transduction as they lack expression of the coxsackie/adenovirus receptor (CAR). To circumvent this obstacle
Kenneth J Mandell et al.
Investigative ophthalmology & visual science, 48(9), 3928-3936 (2007-08-29)
Junctional adhesion molecules (JAMs) are a family of adhesion proteins found in intercellular junctions. Evidence suggests that JAM-A is important for the regulation of tight junction assembly and epithelial barrier function. The authors recently reported that JAM-A is expressed in
Anton V Borovjagin et al.
PloS one, 6(10), e24281-e24281 (2011-10-18)
To explore gene therapy strategies for amelogenesis imperfecta (AI), a human ameloblast-like cell population was established from third molars of an AI-affected patient. These cells were characterized by expression of cytokeratin 14, major enamel proteins and alkaline phosphatase staining. Suboptimal
William C Adams et al.
The Journal of general virology, 90(Pt 7), 1600-1610 (2009-03-14)
The coxsackievirus-adenovirus receptor (CAR) is the described primary receptor for adenovirus serotype 5 (Ad5), a common human pathogen that has been exploited as a viral vector for gene therapy and vaccination. This study showed that monocytes and dendritic cells (DCs)
Antitumor effects of bladder cancer-specific adenovirus carrying E1A-androgen receptor in bladder cancer.
Zhai, Z; Wang, Z; Fu, S; Lu, J; Wang, F; Li, R; Zhang, H; Li, S; Hou, Z; Wang, H; Rodriguez, R
Gene Therapy null
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